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https://hdl.handle.net/2440/17456
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dc.contributor.author | Kortesidis, A. | - |
dc.contributor.author | Zannettino, A. | - |
dc.contributor.author | Isenmann, S. | - |
dc.contributor.author | Shi, S. | - |
dc.contributor.author | Lapidot, T. | - |
dc.contributor.author | Gronthos, S. | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Blood, 2005; 105(10):3793-3801 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.issn | 1528-0020 | - |
dc.identifier.uri | http://hdl.handle.net/2440/17456 | - |
dc.description.abstract | The maintenance of bone marrow stromal stem cells (BMSSCs) is tightly controlled by the local microenvironment and by autocrine regulatory factors secreted by BMSSCs. To identify such factors, a cDNA subtraction library was generated from purified BMSSCs, based on their high expression of the STRO-1 antigen. Stromal-derived factor-1 (SDF-1) was one differentially expressed gene highly expressed by purified BMSSCs prior to culture. In vitro, immature preosteogenic cells expressed greater levels of SDF-1 when compared with mature cell types representative of osteoblasts and osteocytes/bone lining cells. Furthermore, SDF-1 expression was rapidly down-regulated when BMSSCs were cultured under osteoinductive conditions. BMSSCs were also shown to express functional cell surface SDF-1 receptors (CXCR4). Transduced BMSSC lines, secreting high SDF-1 levels, displayed an enhanced ability to form ectopic bone in vivo, in comparison with control BMSSC lines. Moreover, high SDF-1-expressing BMSSCs displayed an increased capacity for cellular growth and protection against interleukin-4-induced apoptosis. Similarly, fibroblast colony-forming units (CFU-Fs) also displayed increased growth and resistance to alpha-interferon-2a-induced apoptosis, in synergy with platelet-derived growth factor BB (PDGF-BB) and SDF-1 in vitro. These studies indicate that the chemokine, SDF-1, may play a role in the maintenance, survival, and osteogenic capacity of immature BMSSC populations. | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Hematology | - |
dc.source.uri | http://dx.doi.org/10.1182/blood-2004-11-4349 | - |
dc.subject | Bone and Bones | - |
dc.subject | Bone Marrow Cells | - |
dc.subject | Stromal Cells | - |
dc.subject | Humans | - |
dc.subject | Receptors, CXCR4 | - |
dc.subject | Chemokines, CXC | - |
dc.subject | Cell Differentiation | - |
dc.subject | Cell Proliferation | - |
dc.subject | Cell Survival | - |
dc.subject | Osteogenesis | - |
dc.subject | Adult | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.subject | Chemokine CXCL12 | - |
dc.title | Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal stem cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1182/blood-2004-11-4349 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Zannettino, A. [0000-0002-6646-6167] | - |
dc.identifier.orcid | Gronthos, S. [0000-0002-6225-3084] | - |
Appears in Collections: | Aurora harvest 6 Medicine publications |
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