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https://hdl.handle.net/2440/17946
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Type: | Journal article |
Title: | The solution structures and activity of caerin 1.1 and caerin 1.4 in aqueous trifluoroethanol and dodecylphosphocholine micelles |
Author: | Wegener, K. Carver, J. Bowie, J. |
Citation: | Biopolymers, 2003; 69(1):42-59 |
Publisher: | John Wiley & Sons Inc |
Issue Date: | 2003 |
ISSN: | 0006-3525 1097-0282 |
Statement of Responsibility: | Kate L. Wegener, John A. Carver and John H. Bowie |
Abstract: | The caerin 1 peptides are among the most powerful of the broad-spectrum antibiotic amphibian peptides. Caerin 1.1 has previously been shown to form an amphipathic helix-bend-helix structure in aqueous trifluoroethanol (H. Wong, J. H. Bowie, and J. A. Carver European Journal Biochemistry, 1997, Vol. 247, pp. 545-557) and structure-activity relationship studies indicate that both helices are required for activity, as well as flexibility in the bend region connecting the two. The structure of caerin 1.1 in dodecylphosphocholine micelles was investigated and shown to be very similar to that determined in aqueous trifluoroethanol. Caerin 1.4, which is identical to caerin 1.1, but with serine residues replacing Val5 and Gly7, is less active than caerin 1.1 against most bacterial species but has improved activity against Escherichia coli and Micrococcus luteus. The solution NMR structure of caerin 1.4 was determined in both aqueous trifluoroethanol and dodecylphosphocholine micelles, and was shown to be similar to caerin 1.1. It was concluded that differences in the hydrophobicity and hydrophilic angle of the first helix are probably responsible for the different spectra of antibacterial activity. The similarity of the structures calculated in aqueous trifluoroethanol and dodecylphosphocholine micelles suggests that, for caerin 1.1 and 1.4, these solvent systems are equally as good at representing a membrane environment. |
Keywords: | amphipathic helix NMR spectroscopy solution structure antimicrobial peptide |
Description: | Copyright © 2003 Wiley Periodicals The definitive version may be found at www.wiley.com |
Provenance: | Published Online: 25 Mar 2003 |
DOI: | 10.1002/bip.10324 |
Published version: | http://www3.interscience.wiley.com/journal/104086264/abstract |
Appears in Collections: | Aurora harvest 2 Chemistry publications |
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