Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/229
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Type: Journal article
Title: An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation
Author: Jacobs, A.
Lipka, V.
Burton, R.
Panstruga, R.
Strizhov, N.
Schulze-Lefert, P.
Fincher, G.
Citation: The Plant Cell, 2003; 15(11):2503-2513
Publisher: Amer Soc Plant Physiologists
Issue Date: 2003
ISSN: 1040-4651
1532-298X
Statement of
Responsibility: 
Andrew K. Jacobs, Volker Lipka, Rachel A. Burton, Ralph Panstruga, Nicolai Strizhov, Paul Schulze-Lefert and Geoffrey B. Fincher
Abstract: Arabidopsis was transformed with double-stranded RNA interference (dsRNAi) constructs designed to silence three putative callose synthase genes: GLUCAN SYNTHASE–LIKE5 (GSL5), GSL6, and GSL11. Both wound callose and papillary callose were absent in lines transformed with GSL5 dsRNAi and in a corresponding sequence-indexed GSL5 T-DNA insertion line but were unaffected in GSL6 and GSL11 dsRNAi lines. These data provide strong genetic evidence that the GSL genes of higher plants encode proteins that are essential for callose formation. Deposition of callosic plugs, or papillae, at sites of fungal penetration is a widely recognized early response of host plants to microbial attack and has been implicated in impeding entry of the fungus. Depletion of callose from papillae in gsl5 plants marginally enhanced the penetration of the grass powdery mildew fungus Blumeria graminis on the nonhost Arabidopsis. Paradoxically, the absence of callose in papillae or haustorial complexes correlated with the effective growth cessation of several normally virulent powdery mildew species and of Peronospora parasitica.
Keywords: Fungi
Plants, Genetically Modified
Arabidopsis
Plant Leaves
Glucans
Glucosyltransferases
Schizosaccharomyces pombe Proteins
Membrane Proteins
DNA, Bacterial
RNA, Double-Stranded
Signal Transduction
Plant Diseases
RNA Interference
Mutation
Stress, Mechanical
Immunity, Innate
Fungal Structures
DOI: 10.1105/tpc.016097
Published version: http://dx.doi.org/10.1105/tpc.016097
Appears in Collections:Agriculture, Food and Wine publications
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