Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23156
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Glucosamine supplementation during in vitro maturation inhibits subsequent embryo development: Possible role of the hexosamine pathway as a regulator of developmental competence.
Author: McDowall, M.
Mitchell, M.
Cetica, P.
Dalvit, G.
Pantaleon, M.
Lane, M.
Gilchrist, R.
Thompson, J.
Citation: Biology of Reproduction, 2006; 74(5):881-888
Publisher: Soc Study Reproduction
Issue Date: 2006
ISSN: 0006-3363
1529-7268
Statement of
Responsibility: 
Melanie L. Sutton-McDowall, Megan Mitchell, Pablo Cetica, Gabriel Dalvit, Marie Pantaleon, Michelle Lane, Robert B. Gilchrist and Jeremy G. Thompson
Abstract: Glucose concentration during cumulus-oocyte complex (COC) maturation influences several functions, including progression of oocyte meiosis, oocyte developmental competence, and cumulus mucification. Glucosamine (GlcN) is an alternative hexose substrate, specifically metabolized through the hexosamine biosynthesis pathway, which provides the intermediates for extracellular matrix formation during cumulus cell mucification. The aim of this study was to determine the influence of GlcN on meiotic progression and oocyte developmental competence following in vitro maturation (IVM). The presence of GlcN during bovine IVM did not affect the completion of nuclear maturation and early cleavage, but severely perturbed blastocyst development. This effect was subsequently shown to be dose-dependent and was also observed for porcine oocytes matured in vitro. Hexosamine biosynthesis upregulation using GlcN supplementation is well known to increase O-linked glycosylation of many intracellular signaling molecules, the bestcharacterized being the phosphoinositol-3-kinase (PI3K) signaling pathway. We observed extensive O-linked glycosylation in bovine cumulus cells, but not oocytes, following IVM in either the presence or the absence of GlcN. Inhibition of O-linked glycosylation significantly reversed the effect of GlcN-induced reduction in developmental competence, but inhibition of PI3K signaling had no effect. Our data are the first to link hexosamine biosynthesis, involved in cumulus cell mucification, to oocyte developmental competence during in vitro maturation.
Keywords: cumulus; cumulus cells; embryo; early development; glucose; glucosamine; hexosamine; oocyte; oocyte development
Description: © 2006 by the Society for the Study of Reproduction, Inc.
RMID: 0020060429
DOI: 10.1095/biolreprod.105.048553
Published version: http://www.biolreprod.org/cgi/content/abstract/74/5/881
Appears in Collections:Obstetrics and Gynaecology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.