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Type: Journal article
Title: Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans
Author: Burton, R.
Wilson, S.
Hrmova, M.
Harvey, A.
Shirley, N.
Medhurst, A.
Stone, B.
Newbigin, E.
Bacic, A.
Fincher, G.
Citation: Science, 2006; 311(5769):1940-1942
Publisher: Amer Assoc Advancement Science
Issue Date: 2006
ISSN: 0036-8075
Statement of
Rachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher
Abstract: A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis.
Keywords: Cell Wall; Plants, Genetically Modified; Arabidopsis; Hordeum; Oryza sativa; Plant Leaves; Glucosyltransferases; beta-Glucans; Antibodies, Monoclonal; Genomics; Transformation, Genetic; Synteny; Genes, Plant; Genome, Plant; Quantitative Trait Loci
RMID: 0020060056
DOI: 10.1126/science.1122975
Appears in Collections:Agriculture, Food and Wine publications

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