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|Title:||Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans|
|Citation:||Science, 2006; 311(5769):1940-1942|
|Publisher:||Amer Assoc Advancement Science|
|Rachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher|
|Abstract:||A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis.|
|Keywords:||Cell Wall; Plants, Genetically Modified; Arabidopsis; Hordeum; Oryza sativa; Plant Leaves; Glucosyltransferases; beta-Glucans; Antibodies, Monoclonal; Genomics; Transformation, Genetic; Synteny; Genes, Plant; Genome, Plant; Quantitative Trait Loci|
|Appears in Collections:||Agriculture, Food and Wine publications|
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