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dc.contributor.authorBurton, R.en
dc.contributor.authorWilson, S.en
dc.contributor.authorHrmova, M.en
dc.contributor.authorHarvey, A.en
dc.contributor.authorShirley, N.en
dc.contributor.authorMedhurst, A.en
dc.contributor.authorStone, B.en
dc.contributor.authorNewbigin, E.en
dc.contributor.authorBacic, A.en
dc.contributor.authorFincher, G.en
dc.identifier.citationScience, 2006; 311(5769):1940-1942en
dc.description.abstractA characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis.en
dc.description.statementofresponsibilityRachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincheren
dc.publisherAmer Assoc Advancement Scienceen
dc.subjectCell Wall; Plants, Genetically Modified; Arabidopsis; Hordeum; Oryza sativa; Plant Leaves; Glucosyltransferases; beta-Glucans; Antibodies, Monoclonal; Genomics; Transformation, Genetic; Synteny; Genes, Plant; Genome, Plant; Quantitative Trait Locien
dc.titleCellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucansen
dc.typeJournal articleen
pubs.library.collectionAgriculture, Food and Wine publicationsen
dc.identifier.orcidBurton, R. [0000-0002-0638-4709]en
dc.identifier.orcidHrmova, M. [0000-0002-3545-0605]en
dc.identifier.orcidShirley, N. [0000-0001-8114-9891]en
Appears in Collections:Agriculture, Food and Wine publications

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