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https://hdl.handle.net/2440/27325
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Type: | Journal article |
Title: | Microfibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique |
Author: | Henderson, M. Polewski, R. Fanning, J. Gibson, M. |
Citation: | Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 1996; 44(12):1389-1397 |
Publisher: | Histochemical Society |
Issue Date: | 1996 |
ISSN: | 0022-1554 1551-5044 |
Statement of Responsibility: | M Henderson, R Polewski, J C Fanning and M A Gibson |
Abstract: | This study used immunoelectron microscopic techniques to define the ultrastructural location of MAGP-1 on the fibrillin-containing microfibrils of the ocular zonule. A specific anti-MAGP-1 monoclonal antibody (MAb), 11B, was produced that did not crossreact with fibrillin-1 or other microfibrillar proteins. MAb 11B was shown by immunofluorescence to localize intensely to zonular tissue. Postembedding immunoelectron microscopy showed that MAGP-1 was associated with microfibrils throughout the zonule, with the exception of a narrow band of microfibrils at the junction with the lens capsule. With preembedding labeling, the anti-MAGP-1 MAb was found to localize in a crossbanding pattern, at intervals of about 50 nm, to microfibrils throughout the zonule and along bundles of microfibrils in surrounding vitreous tissue. Rotary shadowing of isolated microfibrils showed a "beads on a string" morphology with a periodicity of about 50 nm. With immunogold labeling, the anti-MAGP-1 antibody specifically localized on the beads in a symmetrical manner. Occasionally two gold partides were attached to the same bead, suggesting that multiple MAGP-1 molecules were present in the structure. The results indicate that MAGP-1 is intimately and regularly associated with the bead regions of fibrillin-containing microfibrils. The findings are consistent with a major structural role for MAGP-1 in microfibril biology. |
Keywords: | Eye Animals Cattle Microfilament Proteins Contractile Proteins Antibodies, Monoclonal Microscopy, Electron Fluorescent Antibody Technique Antibody Specificity Fibrillins |
Rights: | ©1996 by The Histochemical Society, Inc. |
DOI: | 10.1177/44.12.8985131 |
Published version: | http://dx.doi.org/10.1177/44.12.8985131 |
Appears in Collections: | Adelaide Microscopy publications Aurora harvest 2 |
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