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|Title:||Mixed subunit capsids can be assembled in vitro with coat protein subunits from two cucumoviruses|
Randles, John Wesley
Francki, R. I. B.
|Citation:||Journal of General Virology, 1995; 76(4):971-973|
|Publisher:||Society for General Microbiology|
|School/Discipline:||School of Agriculture, Food and Wine : Plant and Food Science|
School of Agriculture, Food and Wine
|B. Chen, J. W. Randles and R. I. B. Francki|
|Abstract:||Virus particles were reassembled in vitro from tomato aspermy virus strain V (V-TAV) RNA and a mixture of subunits prepared from V-TAV and 35S-labelled cucumber mosaic virus strain T (T-CMV). Immunodiffusion tests showed that the reassembled particles reacted with polyclonal antisera raised against both V-TAV and T-CMV. Radioactivity was found in the precipitin line formed between the reassembled particles and antiserum raised against T-CMV as well as in the precipitin line formed between the reassembled particles and antiserum raised against V-TAV. This shows that 35S-labelled T-CMV protein subunits were incorporated with V-TAV protein subunits into the same particles. Thus, coat proteins of V-TAV and T-CMV can coassemble and form mixed-subunit capsids in vitro.|
|Rights:||© 1995 SGM|
|Appears in Collections:||Agriculture, Food and Wine publications|
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