Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27884
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Type: Journal article
Title: Mixed subunit capsids can be assembled in vitro with coat protein subunits from two cucumoviruses
Author: Chen, Bao-Dong
Randles, John Wesley
Francki, R. I. B.
Citation: Journal of General Virology, 1995; 76(4):971-973
Publisher: Society for General Microbiology
Issue Date: 1995
ISSN: 0022-1317
School/Discipline: School of Agriculture, Food and Wine : Plant and Food Science
School of Agriculture, Food and Wine
Statement of
Responsibility: 
B. Chen, J. W. Randles and R. I. B. Francki
Abstract: Virus particles were reassembled in vitro from tomato aspermy virus strain V (V-TAV) RNA and a mixture of subunits prepared from V-TAV and 35S-labelled cucumber mosaic virus strain T (T-CMV). Immunodiffusion tests showed that the reassembled particles reacted with polyclonal antisera raised against both V-TAV and T-CMV. Radioactivity was found in the precipitin line formed between the reassembled particles and antiserum raised against T-CMV as well as in the precipitin line formed between the reassembled particles and antiserum raised against V-TAV. This shows that 35S-labelled T-CMV protein subunits were incorporated with V-TAV protein subunits into the same particles. Thus, coat proteins of V-TAV and T-CMV can coassemble and form mixed-subunit capsids in vitro.
Rights: © 1995 SGM
DOI: 10.1099/0022-1317-76-4-971
Appears in Collections:Agriculture, Food and Wine publications

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