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Type: Journal article
Title: Altered kinetics and benzodiazepine sensitivity of a GABAA receptor subunit mutation [g2(R43Q)] found in human epilepsy
Author: Bowser, D.
Wagner, D.
Czajkowski, C.
Cromer, B.
Parker, M.
Wallace, R.
Harkin, L.
Mulley, J.
Marini, C.
Berkovic, S.
Williams, D.
Jones, M.
Petrou, S.
Citation: Neuroscience, 2002; 99(23):15170-15175
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2002
ISSN: 0306-4522
Statement of
David N. Bowser, David A. Wagner, Cynthia Czajkowski, Brett A. Cromer, Michael W. Parker, Robyn H. Wallace, Louise A. Harkin, John C. Mulley, Carla Marini, Samuel F. Berkovic, David A. Williams, Mathew V. Jones and Steven Petrou
Abstract: The gamma-aminobutyric acid type A (GABA(A)) receptor mediates fast inhibitory synaptic transmission in the CNS. Dysfunction of the GABA(A) receptor would be expected to cause neuronal hyperexcitability, a phenomenon linked with epileptogenesis. We have investigated the functional consequences of an arginine-to-glutamine mutation at position 43 within the GABA(A) gamma(2)-subunit found in a family with childhood absence epilepsy and febrile seizures. Rapid-application experiments performed on receptors expressed in HEK-293 cells demonstrated that the mutation slows GABA(A) receptor deactivation and increases the rate of desensitization, resulting in an accumulation of desensitized receptors during repeated, short applications. In Xenopus laevis oocytes, two-electrode voltage-clamp analysis of steady-state currents obtained from alpha(1)beta(2)gamma(2) or alpha(1)beta(2)gamma(2)(R43Q) receptors did not reveal any differences in GABA sensitivity. However, differences in the benzodiazepine pharmacology of mutant receptors were apparent. Mutant receptors expressed in oocytes displayed reduced sensitivity to diazepam and flunitrazepam but not the imidazopyridine zolpidem. These results provide evidence of impaired GABA(A) receptor function that could decrease the efficacy of transmission at inhibitory synapses, possibly generating a hyperexcitable neuronal state in thalamocortical networks of epileptic patients possessing the mutant subunit.
Keywords: rapid agonist application; two-electrode voltage clamp; Xenopus oocytes
RMID: 0020021898
DOI: 10.1073/pnas.212320199
Appears in Collections:Molecular and Biomedical Science publications

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