Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/35130
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Type: Journal article
Title: Incorporating historical museum specimens into molecular systematic and conservation genetics research
Author: Austin, J.
Melville, J.
Citation: Molecular Ecology Notes, 2006; 6(4):1089-1092
Publisher: Blackwell Publishing Ltd.
Issue Date: 2006
ISSN: 1471-8286
1471-8286
Statement of
Responsibility: 
Jeremy J. Austin and Jane Melville
Abstract: We successfully amplified mitochondrial DNA (mtDNA) and microsatellites from historical museum specimens of lizards and frogs dating from 1894 through to 1998. Ancient DNA techniques were used to extract whole genomic DNA from a number of different tissues, including liver, leg muscle and toe clips, followed by standard polymerase chain reaction techniques for amplification. We had a 78% success rate amplifying mtDNA from 14 museum specimens and a 57% success rate amplifying microsatellite markers for seven museum specimens. Our study demonstrates the feasibility of incorporating historical museum specimens into molecular systematic and conservation genetic studies.
Keywords: Litoria; molecular systematics; museum specimens; Tympanocryptis
RMID: 0020063025
DOI: 10.1111/j.1471-8286.2006.01443.x
Appears in Collections:Earth and Environmental Sciences publications
Australian Centre for Ancient DNA publications
Environment Institute Leaders publications

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