Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/35130
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dc.contributor.authorAustin, J.en
dc.contributor.authorMelville, J.en
dc.date.issued2006en
dc.identifier.citationMolecular Ecology Notes, 2006; 6(4):1089-1092en
dc.identifier.issn1471-8286en
dc.identifier.issn1471-8286en
dc.identifier.urihttp://hdl.handle.net/2440/35130-
dc.description.abstractWe successfully amplified mitochondrial DNA (mtDNA) and microsatellites from historical museum specimens of lizards and frogs dating from 1894 through to 1998. Ancient DNA techniques were used to extract whole genomic DNA from a number of different tissues, including liver, leg muscle and toe clips, followed by standard polymerase chain reaction techniques for amplification. We had a 78% success rate amplifying mtDNA from 14 museum specimens and a 57% success rate amplifying microsatellite markers for seven museum specimens. Our study demonstrates the feasibility of incorporating historical museum specimens into molecular systematic and conservation genetic studies.en
dc.description.statementofresponsibilityJeremy J. Austin and Jane Melvilleen
dc.language.isoenen
dc.publisherBlackwell Publishing Ltd.en
dc.subjectLitoria; molecular systematics; museum specimens; Tympanocryptisen
dc.titleIncorporating historical museum specimens into molecular systematic and conservation genetics researchen
dc.typeJournal articleen
dc.identifier.rmid0020063025en
dc.identifier.doi10.1111/j.1471-8286.2006.01443.xen
dc.identifier.pubid51187-
pubs.library.collectionEarth and Environmental Sciences publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidAustin, J. [0000-0003-4244-2942]en
Appears in Collections:Earth and Environmental Sciences publications
Australian Centre for Ancient DNA publications
Environment Institute Leaders publications

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