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Type: Journal article
Title: Cannabinoid 1 (CB1) receptors coupled to cholinergic motorneurones inhibit neurogenic circular muscle contractility in the human colon
Author: Hinds, N.
Ullrich, K.
Smid, S.
Citation: British Journal of Pharmacology, 2006; 148(2):191-199
Publisher: Nature Publishing Group
Issue Date: 2006
ISSN: 0007-1188
Statement of
Nicholas M. Hinds, Katja Ullrich & Scott D. Smid
Abstract: The effects of cannabinoid subtype 1 (CB(1)) receptor activation were determined on smooth muscle, inhibitory and excitatory motorneuronal function in strips of human colonic longitudinal muscle (LM) and circular muscle (CM) in vitro. Electrical field stimulation (EFS; 0.5-20 Hz, 50 V) evoked a relaxation in LM and CM precontracted with a neurokinin-2 (NK-2) selective receptor agonist (beta-ala(8)-neurokinin A; 10(-6) M) in the presence of atropine (10(-6) M); this was unaltered following pretreatment with the CB(1)-receptor selective agonist arachidonyl-2-chloroethylamide (ACEA; 10(-6) M). In the presence of nitric oxide synthase blockade with N-nitro-L-arginine (10(-4) M), EFS evoked a frequency-dependent 'on-contraction' during stimulation and an 'off-contraction' following stimulus cessation. On-contractions were significantly inhibited in CM strips by pretreatment with ACEA (10(-6) M). These inhibitory effects were reversed in the presence of the CB(1) receptor-selective antagonist N-(piperidine-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (10(-7) M). ACEA did not alter LM or CM contractile responses to acetylcholine or NK-2 receptor-evoked contraction. Immunohistochemical studies revealed a colocalisation of CB(1) receptors to cholinergic neurones in the human colon based on colabelling with choline acetyltransferase, in addition to CB(1) receptor labelling in unidentified structures in the CM. In conclusion, activation of CB(1) receptors coupled to cholinergic motorneurones selectively and reversibly inhibits excitatory nerve transmission in colonic human colonic CM. These results provide evidence of a direct role for cannabinoids in the modulation of motor activity in the human colon by coupling to cholinergic motorneurones.
Keywords: Cannabinoid, colonic motility, cholinergic, smooth muscle, enteric motorneurones, arachidonyl-2-chloroethylamide
Description: Copyright © 2006 Nature Publishing Group
RMID: 0020065531
DOI: 10.1038/sj.bjp.0706710
Appears in Collections:Pharmacology publications

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