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Type: Journal article
Title: A technique for preserving pigmentation in some capsalid monogeneans for taxonomic purposes
Author: Deveney, M.
Whittington, I.
Citation: Systematic Parasitology, 2001; 48(1):31-35
Publisher: Kluwer Academic Publ
Issue Date: 2001
ISSN: 0165-5752
Statement of
Marty R. Deveney and Ian D. Whittington
Abstract: A technique is described to preserve the pigment found in the bodies and the intestine of some brightly coloured and darkly pigmented benedeniine capsalid monogeneans. Previous studies of these pigmented capsalids have proven difficult because the pigmentation usually disappears when the worms are fixed using preservatives containing concentrations of formalin over 5% and/or ethanol, acetic acid, chromic acid, picric acid and mercuric chloride. The technique developed here uses a fixative comprising glycerol, acetone and formalin (GAF). After fixation under light coverslip compression for three minutes, specimens are transferred to absolute acetone for three minutes and cleared in a mixture of nine parts cedar wood oil and one part absolute acetone before mounting in Canada balsam. Processing must be carried out quickly, as these chemicals will cause the pigments to fade if the specimens are exposed to them for too long. Pigmented benedeniines processed using this technique retain the distribution, intensity and colour observed in live worms. The colour and distribution of pigmentation in monogeneans may be of taxonomic importance and this technique aids preparation of whole-mounts suitable for registration as type-material.
Keywords: Animals; Fishes; Trematoda; Glycerol; Formaldehyde; Acetone; Balsams; Oils, Volatile; Tissue Fixation; Pigments, Biological
Description: The original publication can be found at
RMID: 0020071393
DOI: 10.1023/A:1026558405617
Appears in Collections:Earth and Environmental Sciences publications
Environment Institute publications

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