Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||A technique for preserving pigmentation in some capsalid monogeneans for taxonomic purposes|
|Citation:||Systematic Parasitology, 2001; 48(1):31-35|
|Publisher:||Kluwer Academic Publ|
|Marty R. Deveney and Ian D. Whittington|
|Abstract:||A technique is described to preserve the pigment found in the bodies and the intestine of some brightly coloured and darkly pigmented benedeniine capsalid monogeneans. Previous studies of these pigmented capsalids have proven difficult because the pigmentation usually disappears when the worms are fixed using preservatives containing concentrations of formalin over 5% and/or ethanol, acetic acid, chromic acid, picric acid and mercuric chloride. The technique developed here uses a fixative comprising glycerol, acetone and formalin (GAF). After fixation under light coverslip compression for three minutes, specimens are transferred to absolute acetone for three minutes and cleared in a mixture of nine parts cedar wood oil and one part absolute acetone before mounting in Canada balsam. Processing must be carried out quickly, as these chemicals will cause the pigments to fade if the specimens are exposed to them for too long. Pigmented benedeniines processed using this technique retain the distribution, intensity and colour observed in live worms. The colour and distribution of pigmentation in monogeneans may be of taxonomic importance and this technique aids preparation of whole-mounts suitable for registration as type-material.|
|Keywords:||Animals; Fishes; Trematoda; Glycerol; Formaldehyde; Acetone; Balsams; Oils, Volatile; Tissue Fixation; Pigments, Biological|
|Description:||The original publication can be found at www.springerlink.com|
|Appears in Collections:||Earth and Environmental Sciences publications|
Environment Institute publications
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.