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Type: Journal article
Title: Molecular evolution of the carboxy terminal region of the zona pellucida 3 glycoprotein in murine rodents
Author: Swann, C.
Cooper, S.
Breed, W.
Citation: Reproduction, 2007; 133(4):697-708
Publisher: Bio Scientifica Ltd
Issue Date: 2007
ISSN: 1470-1626
Statement of
Christine A Swann, Steven J B Cooper and William G Breed
Abstract: In mammals, before fertilization can occur, sperm have to bind to, and penetrate, the zona pellucida (ZP). In the laboratory mouse, which has been used as a model system for fertilization studies, sperm-ZP binding has been found to be mediated by a region at the carboxy terminal, encoded by exon 7 of the Zp3 gene. This region shows considerable interspecific sequence diversity with some evidence of adaptive evolution in mammals, suggesting that it may contribute to species-specific sperm-ZP binding. However, in a previous study of sequence diversity of ZP3 of three species of Australian murine rodents, we found an identical protein sequence of the region encoded by exon 7. Here, we expand this earlier study to determine the sequence diversity of this region in 68 out of the 130 species of Australasian murine rodents. Maximum likelihood analyses, using representatives of both New Guinean and Australian taxa, provide evidence of positive selection at three codons adjacent to, or within, the putative combining-site for sperm of ZP3, but this was not evident when the analysis was restricted to the Australian taxa. The latter group showed low levels of both intra- and inter-generic sequence divergences in the region encoded by exon 7 of Zp3, with little evidence that this region contributes to species specificity of sperm-ZP binding. These findings suggest that the selective forces acting on the Zp3 exon 7 region during the evolution of the Australasian murine rodents have been variable, and that positive selection has only occurred in a few lineages.
Keywords: Animals
Egg Proteins
Membrane Glycoproteins
Receptors, Cell Surface
Sequence Analysis, DNA
Evolution, Molecular
Species Specificity
Base Sequence
Protein Structure, Tertiary
Sperm-Ovum Interactions
Molecular Sequence Data
Selection, Genetic
Zona Pellucida Glycoproteins
Description: Copyright © 2007 Society for Reproduction and Fertility
DOI: 10.1530/REP-06-0043
Published version:
Appears in Collections:Anatomical Sciences publications
Aurora harvest
Environment Institute Leaders publications
Environment Institute publications

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