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https://hdl.handle.net/2440/44101
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Type: | Journal article |
Title: | MALDI-TOF mass spectometry for multiplex genotyping of CYP2B6 single-nucleotide polymorphisms |
Author: | Blievernicht, J. Schaeffeler, E. Klein, K. Eichelbaum, M. Schwab, M. Zanger, U. |
Citation: | Clinical Chemistry (Washington, DC): international journal of molecular diagnostics and laboratory medicine, 2007; 53(1):24-33 |
Publisher: | Amer Assoc Clinical Chemistry |
Issue Date: | 2007 |
ISSN: | 0009-9147 1530-8561 |
Statement of Responsibility: | Julia K. Blievernicht, Elke Schaeffeler, Kathrin Klein, Michel Eichelbaum, Matthias Schwab and Ulrich M. Zanger |
Abstract: | Background: CYP2B6 is a highly variable and polymorphic cytochrome P450 (CYP) enzyme involved in the biotransformation of an increasing number of drugs, including cyclophosphamide, bupropion, and the nonnucleosidic reverse transcriptase inhibitor efavirenz. Several nonsynonymous and promoter single-nucleotide polymorphisms (SNPs) in the CYP2B6 gene are associated with altered hepatic expression and function, which affect drug plasma concentrations. Methods: We used multiplex PCR to amplify relevant gene fragments while avoiding amplification of the CYP2B7P1 pseudogene. Polymorphic sites were analyzed by allele-specific primer extension followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Method evaluation was performed on a panel of 287 genomic DNA samples previously genotyped by other methods. Results: Five multiplex assays were developed, comprising the following 15 SNPs: –82TC (*22); 86GC (R29T, *17); 136AG (M46V, *11); 296GA (G99E, *12); 415AG (K139E, *8, *13); 419GA (R140Q, *14); 516GT (Q172H, *6, *7, *9, *13, *19, *20), 547GA (V183I); 769GA (D257N); 785AG (K262R, *4, *6, *7, *13, *16, *19, *20); 983TC (I328T, *16, *18); 1006CT (R336C, *19); 1172TA (I391N, *15); 1282CA (P428T, *21); 1459CT (R487C, *5, *7). In 9 DNA samples showing discrepant genotypes, correctness of the MALDI-TOF MS result was confirmed by direct sequencing. Conclusions: This genotyping method enabled sensitive, specific, accurate, and comprehensive determination of 15 relevant SNPs of CYP2B6. The assay design allows analysis of SNP subsets, incorporation of additional SNPs, and performance of high-throughput genotyping. |
Keywords: | Humans Aryl Hydrocarbon Hydroxylases Oxidoreductases, N-Demethylating Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Polymerase Chain Reaction Genotype Polymorphism, Single Nucleotide Cytochrome P-450 CYP2B6 White People |
Description: | Copyright © 2007 by the American Association for Clinical Chemistry. |
DOI: | 10.1373/clinchem.2006.074856 |
Published version: | http://dx.doi.org/10.1373/clinchem.2006.074856 |
Appears in Collections: | Aurora harvest Pathology publications |
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