Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/44503
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPringle, K.en
dc.contributor.authorKind, K.en
dc.contributor.authorThompson, J.en
dc.contributor.authorRoberts, C.en
dc.date.issued2007en
dc.identifier.citationPlacenta, 2007; 28(11-12):1147-1157en
dc.identifier.issn0143-4004en
dc.identifier.issn1532-3102en
dc.identifier.urihttp://hdl.handle.net/2440/44503-
dc.descriptionCopyright © 2007 Elsevier Ltd All rights reserved.en
dc.description.abstractThe human first trimester placenta experiences a low oxygen environment. The hypoxia inducible factors (HIFs) mediate the response to low oxygen, inducing genes such as insulin-like growth factor (IGF)-II. Interestingly, IGF-II has been shown to promote placental growth and function. Currently, the interaction between oxygen, IGF-II and HIFs in the regulation of trophoblast behaviour are unclear. Murine implantation sites from days 5.5-10.5 were collected for immunohistochemical analyses. Use of the hypoxia marker pimonidazole indicated that the early mouse implantation site is exposed to low oxygen levels similar to those seen in the early human placenta. HIF-1alpha protein immunostaining was also observed in the implantation site. Culturing murine ectoplacental cones in decreasing oxygen concentrations (20%, 5% and 1% O(2)), either with or without the addition of IGF-II, induced complex responses by trophoblasts in terms of their migration and differentiation. Following 3 days exposure to low oxygen there was reduced EPC outgrowth, reduced Igf2 and increased Tpbp mRNA levels, suggesting commitment to the spongiotrophoblast lineage. In addition, Hif-1alpha mRNA levels were decreased, whilst Hif-2alpha mRNA was unchanged. This decrease in Hif-1alpha may be due to the observed increase in antisense (as) Hif-1alpha mRNA levels in 1% cultures. Furthermore, expression of Hif-2alpha and the HIF target genes: asHif-1alpha, Vegf and Slc2a1 were reduced under low oxygen with the addition of IGF-II. In conclusion, Hif-1alpha and Hif-2alpha are differentially regulated by oxygen and IGF-II in cultured trophoblast cells and asHif-1alpha may mediate the response to prolonged hypoxia in murine trophoblasts.en
dc.description.statementofresponsibilityK.G. Pringle, K.L. Kind, J.G. Thompson and C.T. Robertsen
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/623064/description#descriptionen
dc.language.isoenen
dc.publisherW B Saunders Co Ltden
dc.subjectDecidua; Cells, Cultured; Trophoblasts; Animals; Mice, Inbred C57BL; Mice; Oxygen; Insulin-Like Growth Factor II; Receptor, IGF Type 2; RNA, Messenger; Biological Markers; Immunohistochemistry; Polymerase Chain Reaction; Cell Differentiation; Cell Hypoxia; Female; Male; Hypoxia-Inducible Factor 1, alpha Subuniten
dc.titleComplex interactions between hypoxia inducible factors, insulin-like growth Factor-II and oxygen in early murine trophoblastsen
dc.typeJournal articleen
dc.identifier.rmid0020073588en
dc.identifier.doi10.1016/j.placenta.2007.05.009en
dc.identifier.pubid46958-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]en
dc.identifier.orcidRoberts, C. [0000-0002-9250-2192]en
Appears in Collections:Obstetrics and Gynaecology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.