Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/4859
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Type: Journal article
Title: A comparison of the positive and negative ion mass spectra of peptides from the dorsal glandular secretion of the Australian red tree frog, Litoria rubella
Author: Bowie, J.
Steinborner, S.
Citation: Rapid Communications in Mass Spectrometry, 1996; 10(10):1243-1247
Publisher: JOHN WILEY & SONS LTD
Issue Date: 1996
ISSN: 0951-4198
1097-0231
Abstract: The collision-induced tandem mass spectral data for MH+ and [M-H]- ions from six bio-active peptides from Litoria rubella are compared. Backbone cleavages of [M-H]- ions provide sequencing information for five of the peptides [e.g. Phe Pro Trp Leu (NH2) and pGlu Phe Pro Trp Leu (NH2)] and in these cases, the negative-ion spectra are as informative as the positive-ion spectra. Side-chain cleavages are also noted in these spectra. For example, (i) when Trp is present, the loss of C9H7N (129 u) competes with the backbone cleavages, and (ii) the [M-H]- ion of Ile Glu Phe Phe Thr (NH2) undergoes facile side-chain fragmentation [loss of H2O (from Glu) and MeCHO (from Thr)], but does not form any conventional backbone-cleavage ions.
Keywords: Animals; Anura; Amino Acids; Peptides; Spectrometry, Mass, Fast Atom Bombardment; Amino Acid Sequence; Molecular Sequence Data; Mass Spectrometry
RMID: 0030002828
DOI: 10.1002/(SICI)1097-0231(19960731)10:10<1243::AID-RCM639>3.0.CO;2-I
Appears in Collections:Chemistry publications

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