Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/49938
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | GRK2 interacts with and phosphorylates Nedd4 and Nedd4-2 |
Author: | Sanchez-Perez, A. Kumar, S. Cook, D. |
Citation: | Biochemical and Biophysical Research Communications, 2007; 359(3):611-615 |
Publisher: | Academic Press Inc |
Issue Date: | 2007 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Angeles Sanchez-Perez, Sharad Kumar and David I. Cook |
Abstract: | Epithelial Na+ channels (ENaC) mediate the transport of sodium (Na) across epithelia in the kidney, gut, and lungs and are required for blood pressure regulation. They are inhibited by ubiquitin protein ligases, such as Nedd4 and Nedd4-2, which bind to proline-rich motifs (PY motifs) present in the C-termini of ENaC subunits. Loss of inhibition leads to hypertension. ENaC channels are maintained in the active state by G-protein-coupled receptor kinase 2 (GRK2), an enzyme implicated in the development of essential hypertension. Here, we report that GRK2 interacts not only with ENaC, but also with both Nedd4 and Nedd4-2. Additionally, GRK2 is capable of phosphorylating both Nedd4 and Nedd4-2 at multiple sites. Of possible significance is the phosphorylation of the threonine at position 466 in Nedd4, which is located in the area of the ww3 domain that binds ENaC. These results support and extend the role of GRK2 in sodium transport regulation. |
Keywords: | Cell Line Humans Ubiquitin-Protein Ligases Amino Acid Sequence Protein Binding Phosphorylation Molecular Sequence Data beta-Adrenergic Receptor Kinases G-Protein-Coupled Receptor Kinase 2 Endosomal Sorting Complexes Required for Transport Nedd4 Ubiquitin Protein Ligases |
Description: | Copyright © 2007 Elsevier |
DOI: | 10.1016/j.bbrc.2007.05.134 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#description |
Published version: | http://dx.doi.org/10.1016/j.bbrc.2007.05.134 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.