Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/5239
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Type: Journal article
Title: Low concentrations of methaemoglobin in marine fishes of the Great Barrier Reef, Australia
Author: Wells, R.
Baldwin, J.
Seymour, R.
Citation: Marine and Freshwater Research, 1997; 48(4):303-309
Publisher: C S I R O PUBLICATIONS
Issue Date: 1997
ISSN: 1323-1650
Statement of
Responsibility: 
Wells, Rufus M. G; Baldwin, John; Seymour, Roger S
Abstract: <jats:p> Concentrations of methaemoglobin (the oxidized non-functional ferric form of haemoglobin) in the blood of marine fish are poorly documented. Although high concentrations have been reported for fish maintained in captivity, baseline values for wild populations are unknown. Two techniques, the cyanide derivative method and the multiple wavelength method, were used to determine methaemoglobin concentrations in blood samples from 25 species of marine teleosts and elasmobranchs captured on the Australian Great Barrier Reef. Although methaemoglobin generally accounted for less than 2% of total haemoglobin, systematic errors occurred when these two standard methods, developed for mammalian blood, were applied to the blood of some fish species. Most problems arose from reactions of various blood components with the reagents used in the cyanide derivative method. Consequently, the multiple wavelength method generally was more reliable for estimating methaemoglobin in the blood of marine fish. The low methaemoglobin concentrations in fish studied on the Great Barrier Reef indicate high water quality and healthy physiological condition.</jats:p>
DOI: 10.1071/MF97024
Appears in Collections:Aurora harvest 5
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