Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/5503
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Altered zinc homeostasis and caspase-3 activity in murine allergic airway inflammation |
Author: | TruongTran, A. Ruffin, R. Foster, P. Koskinen, A. Coyle, P. Philcox, J. Rofe, A. Zalewski, P. |
Citation: | American Journal of Respiratory Cell and Molecular Biology, 2002; 27(3):286-296 |
Publisher: | American Thoracic Society |
Issue Date: | 2002 |
ISSN: | 1044-1549 1535-4989 |
Statement of Responsibility: | Ai Q. Truong-Tran, Richard E. Ruffin, Paul S. Foster, Aulikki M. Koskinen, Peter Coyle, Jeffrey C. Philcox, Allan M Rofe and Peter D. Zalewski |
Abstract: | Zn may have an important protective role in the respiratory epithelium and Zn deficiency may enhance airway inflammation and epithelial damage. The effects of mild nutritional Zn deficiency on airway hyperresponsiveness (AHR) and airway inflammation in mice sensitized and challenged with ovalbumin (OVA) to induce an allergic response were investigated. Balb/c mice were given Zn normal (ZN, 50 mg/kg Zn) or Zn limited diets (ZL, 14 mg/kg Zn) before and during induction of allergic airway inflammation, with appropriate controls (saline- treated, SAL). ZL mice had greater levels of AHR than ZN mice, regardless of presence or absence of allergic inflammation. These mice also had increased eosinophilia and mucus cell hyperplasia compared with ZN mice. Second, ZN and ZL OVA-treated mice had significant decreases in airway epithelial Zinquin fluorescence, indicating a lowered availability of Zn compared with their SAL-treated counterparts. In contrast, the pro-apoptotic protein caspase-3, which was co-localized with Zn in the apical epithelium, was significantly increased in both ZN and ZL OVA-treated mice. Immunologically active caspase-3 and apoptosis were increased in OVA-treated mice, especially the ZL group. These findings provide the first data for adverse effects of Zn deficiency on the respiratory epithelium and support a role for altered Zn homeostasis and caspase upregulation in asthma. |
Keywords: | Liver Respiratory Mucosa Epithelial Cells Animals Mice, Inbred BALB C Mice Respiratory Hypersensitivity Eosinophilia Disease Models, Animal Inflammation Body Weight Zinc Enzyme Precursors Caspases Ovalbumin Apoptosis Homeostasis Dietary Supplements Female Caspase 3 |
Description: | © 2002 American Thoracic Society |
DOI: | 10.1165/rcmb.2001-0014OC |
Published version: | http://ajrcmb.atsjournals.org/cgi/content/abstract/27/3/286 |
Appears in Collections: | Aurora harvest 7 Medicine publications Pathology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.