Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/55471
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Type: Journal article
Title: Protection of pigs from swine dysentery by vaccination with recombinant BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae
Author: La, T.
Phillips, N.
Reichel, M.
Hampson, D.
Citation: Veterinary Microbiology, 2004; 102(1-2):97-109
Publisher: Elsevier Science BV
Issue Date: 2004
ISSN: 0378-1135
Statement of
Responsibility: 
Tom La, Nyree D. Phillips, Michael P. Reichel, David J. Hampson
Abstract: Swine dysentery (SD) is an important endemic infection in many piggeries, and control can be problematic. In this study the efficacy of BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae, was evaluated as an SD vaccine. Non-lipidated BmpB was expressed in Escherichia coli as a histidine-tagged protein (His6-BmpB), or as an 8 kDa carboxy-terminal portion fused to maltose-binding protein (MBP-BmpB-F604). The purified proteins were emulsified with oil-based adjuvants for intramuscular (im) administrations. In experiment 1, 20 weaner pigs were vaccinated im with 1 mg of His6-BmpB. After 3 weeks, 10 received 1 mg of the protein orally (im/oral), and 10 received 1 mg im (im/im). Ten acted as unvaccinated controls. In experiment 2, 12 pigs were vaccinated im with 1 mg of His6-BmpB, and 12 with 1 mg of MBP-BmpB-F604. Three weeks later, each was given 1 mg of the same protein orally. Twelve pigs acted as unvaccinated controls. All pigs were challenged orally with B. hyodysenteriae 2 weeks after their second vaccination. In both experiments, all pigs vaccinated with His6-BmpB developed serum antibodies to BmpB, and oral administration provided boosting of im-induced serum antibody titres. In experiment 1, seven non-vaccinated control pigs developed dysentery and severe colitis. Three pigs vaccinated im/oral developed diarrhoea; two had severe colitis and one had mild lesions. Four pigs vaccinated im/im developed diarrhoea; one had severe colitis and the others had mild lesions. In experiment 2, six control pigs developed SD with severe colitis. Two His6-BmpB vaccinated pigs developed SD with mild colitis. Nine pigs vaccinated with MBP-BmpB-F604 developed SD and severe colitis. Overall, 50–70% of controls and 17–40% of His6-BmpB vaccinated pigs developed disease. Vaccination with MBP-BmpB-F604 did not induce serum titres against BmpB, nor confer protection. The incidence of disease for the three His6-BmpB vaccinated groups was significantly less (P = 0.047) than for the control groups, with a ∼50% reduction. BmpB appears to have potential as an SD vaccine component.
Keywords: Pig-bacteria; Brachyspira hyodysenteriae; BmpB; Lipoprotein; Vaccination; Swine dysentery
Description: Available online 25 July 2004.
Rights: Copyright © 2004 Elsevier B.V. All rights reserved.
RMID: 0020093739
DOI: 10.1016/j.vetmic.2004.06.004
Appears in Collections:Animal and Veterinary Sciences publications

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