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https://hdl.handle.net/2440/55471
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dc.contributor.author | La, T. | - |
dc.contributor.author | Phillips, N. | - |
dc.contributor.author | Reichel, M. | - |
dc.contributor.author | Hampson, D. | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Veterinary Microbiology, 2004; 102(1-2):97-109 | - |
dc.identifier.issn | 0378-1135 | - |
dc.identifier.issn | 1873-2542 | - |
dc.identifier.uri | http://hdl.handle.net/2440/55471 | - |
dc.description | Available online 25 July 2004. | - |
dc.description.abstract | Swine dysentery (SD) is an important endemic infection in many piggeries, and control can be problematic. In this study the efficacy of BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae, was evaluated as an SD vaccine. Non-lipidated BmpB was expressed in Escherichia coli as a histidine-tagged protein (His6-BmpB), or as an 8 kDa carboxy-terminal portion fused to maltose-binding protein (MBP-BmpB-F604). The purified proteins were emulsified with oil-based adjuvants for intramuscular (im) administrations. In experiment 1, 20 weaner pigs were vaccinated im with 1 mg of His6-BmpB. After 3 weeks, 10 received 1 mg of the protein orally (im/oral), and 10 received 1 mg im (im/im). Ten acted as unvaccinated controls. In experiment 2, 12 pigs were vaccinated im with 1 mg of His6-BmpB, and 12 with 1 mg of MBP-BmpB-F604. Three weeks later, each was given 1 mg of the same protein orally. Twelve pigs acted as unvaccinated controls. All pigs were challenged orally with B. hyodysenteriae 2 weeks after their second vaccination. In both experiments, all pigs vaccinated with His6-BmpB developed serum antibodies to BmpB, and oral administration provided boosting of im-induced serum antibody titres. In experiment 1, seven non-vaccinated control pigs developed dysentery and severe colitis. Three pigs vaccinated im/oral developed diarrhoea; two had severe colitis and one had mild lesions. Four pigs vaccinated im/im developed diarrhoea; one had severe colitis and the others had mild lesions. In experiment 2, six control pigs developed SD with severe colitis. Two His6-BmpB vaccinated pigs developed SD with mild colitis. Nine pigs vaccinated with MBP-BmpB-F604 developed SD and severe colitis. Overall, 50–70% of controls and 17–40% of His6-BmpB vaccinated pigs developed disease. Vaccination with MBP-BmpB-F604 did not induce serum titres against BmpB, nor confer protection. The incidence of disease for the three His6-BmpB vaccinated groups was significantly less (P = 0.047) than for the control groups, with a ∼50% reduction. BmpB appears to have potential as an SD vaccine component. | - |
dc.description.statementofresponsibility | Tom La, Nyree D. Phillips, Michael P. Reichel, David J. Hampson | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Science BV | - |
dc.rights | Copyright © 2004 Elsevier B.V. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.vetmic.2004.06.004 | - |
dc.subject | Pig-bacteria | - |
dc.subject | Brachyspira hyodysenteriae | - |
dc.subject | BmpB | - |
dc.subject | Lipoprotein | - |
dc.subject | Vaccination | - |
dc.subject | Swine dysentery | - |
dc.title | Protection of pigs from swine dysentery by vaccination with recombinant BmpB, a 29.7 kDa outer-membrane lipoprotein of Brachyspira hyodysenteriae | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.vetmic.2004.06.004 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 5 |
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