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https://hdl.handle.net/2440/55690
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Type: | Journal article |
Title: | Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle |
Author: | Hall, C. Reichel, M. Ellis, J. |
Citation: | Veterinary Parasitology, 2006; 140(1-2):61-68 |
Publisher: | Elsevier Science BV |
Issue Date: | 2006 |
ISSN: | 0304-4017 1873-2550 |
Statement of Responsibility: | C.A. Hall, M.P. Reichel, J.T. Ellis |
Abstract: | Aim: To determine the performance characteristics of two enzyme-linked immunosorbent assays (ELISAs) manufactured by Institut Pourquier (IP) for the detection of antibodies against Neospora caninum in bovine sera. Methods: Sera from 526 cattle were assayed in two ELISAs (IP) for the detection of anti-N. caninum antibodies. Results from a further ELISA (IDEXX) were used to provide the "gold standard" N. caninum infection status of the cattle and the ELISA results assessed by two-graph receiver operating characteristic (TG-ROC) analysis. Results: TG-ROC analysis suggested changes to one of the IP ELISA protocols, arriving at a cut-off threshold that was different to the one recommended by the manufacturer. With that change, both of the ELISAs performed with high sensitivity and specificity (in excess of 98%) for bovine sera. Conclusions: The analysis of the two IP ELISAs when used on individual bovine sera demonstrated high sensitivity and specificity. TG-ROC analyses optimised the cut-off point suggested by the manufacturer for one of these commercial diagnostic assays and found agreement with the manufacturer's cut-off regarding the other assay. This will help with the accurate identification of infected animals and thereby contributing to the control of neosporosis. © 2006 Elsevier B.V. All rights reserved. |
Keywords: | Neospora caninum Protozoa Cattle Serology ELISA ROC analysis |
Description: | Copyright © 2006 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.vetpar.2006.03.016 |
Published version: | http://dx.doi.org/10.1016/j.vetpar.2006.03.016 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
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