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https://hdl.handle.net/2440/56695
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DC Field | Value | Language |
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dc.contributor.author | McMichael, G. | - |
dc.contributor.author | Gibson, C. | - |
dc.contributor.author | O'Callaghan, M. | - |
dc.contributor.author | Goldwater, P. | - |
dc.contributor.author | Dekker, G. | - |
dc.contributor.author | Haan, E. | - |
dc.contributor.author | MacLennan, A. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Journal of Biomolecular Techniques, 2009; 20(5):232-235 | - |
dc.identifier.issn | 1524-0215 | - |
dc.identifier.issn | 1943-4731 | - |
dc.identifier.uri | http://hdl.handle.net/2440/56695 | - |
dc.description | Copyright © 2009 Association of Biomolecular Resource Facilities | - |
dc.description.abstract | We sought a convenient and reliable method for collection of genetic material that is inexpensive and noninvasive and suitable for self-collection and mailing and a compatible, commercial DNA extraction protocol to meet quantitative and qualitative requirements for high-throughput single nucleotide polymorphism (SNP) multiplex analysis on an automated platform. Buccal swabs were collected from 34 individuals as part of a pilot study to test commercially available buccal swabs and DNA extraction kits. DNA was quantified on a spectrofluorometer with Picogreen dsDNA prior to testing the DNA integrity with predesigned SNP multiplex assays. Based on the pilot study results, the Catch-All swabs and Isohelix buccal DNA isolation kit were selected for our high-throughput application and extended to a further 1140 samples as part of a large cohort study. The average DNA yield in the pilot study (n=34) was 1.94 μg ± 0.54 with a 94% genotyping pass rate. For the high-throughput application (n=1140), the average DNA yield was 2.44 μg ± 1.74 with a ≥93% genotyping pass rate. The Catch-All buccal swabs are a convenient and cost-effective alternative to blood sampling. Combined with the Isohelix buccal DNA isolation kit, they provided DNA of sufficient quantity and quality for high-throughput SNP multiplex analysis. | - |
dc.description.statementofresponsibility | Gai L. McMichael, Catherine S. Gibson, Michael E. O’Callaghan, Paul N. Goldwater, Gustaaf A. Dekker, Eric A. Haan, Alastair H. MacLennan, and for the South Australian Cerebral Palsy Research Group | - |
dc.language.iso | en | - |
dc.publisher | The Association of Biomolecular Resource Facilities | - |
dc.source.uri | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777348/?report=abstract | - |
dc.subject | single nucleotide polymorphism | - |
dc.title | DNA from Buccal Swabs Suitable for High-Throughput SNP Multiplex Analysis | - |
dc.type | Journal article | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McMichael, G. [0000-0002-6811-5301] | - |
dc.identifier.orcid | O'Callaghan, M. [0000-0001-5038-5859] [0000-0002-8178-9714] | - |
dc.identifier.orcid | Goldwater, P. [0000-0003-4822-8488] | - |
dc.identifier.orcid | Dekker, G. [0000-0002-7362-6683] | - |
dc.identifier.orcid | Haan, E. [0000-0002-7310-5124] | - |
Appears in Collections: | Aurora harvest 5 Cerebral Palsy Research Group publications Obstetrics and Gynaecology publications |
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