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https://hdl.handle.net/2440/59751
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Type: | Journal article |
Title: | Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis |
Author: | Ong, Z. Gibson, R. Bowen, J. Stringer, A. Darby, J. Logan, R. Yeoh, A. Keefe, D. |
Citation: | Radiation Oncology, 2010; 5(22):1-8 |
Publisher: | BioMed Central Ltd. |
Issue Date: | 2010 |
ISSN: | 1748-717X 1748-717X |
Statement of Responsibility: | Zhi Yi Ong, Rachel J. Gibson, Joanne M. Bowen, Andrea M. Stringer, Jocelyn M. Darby, Richard M. Logan, Ann S.J. Yeoh, Dorothy M. Keefe |
Abstract: | Background: Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis. However, whether they play a key role in the development of radiotherapy-induced gastrointestinal mucositis is still unknown. Therefore, the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the gastrointestinal tract using a rat model of fractionated radiotherapy-induced toxicity. Methods: Thirty six female Dark Agouti rats were randomly assigned into groups and received 2.5 Gys abdominal radiotherapy three times a week over six weeks. Real time PCR was conducted to determine the relative change in mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF in the jejunum and colon. Protein expression of IL-1β, IL-6 and TNF in the intestinal epithelium was investigated using qualitative immunohistochemistry. Results: Radiotherapy-induced sub-acute damage was associated with significantly upregulated IL-1β, IL-6 and TNF mRNA levels in the jejunum and colon. The majority of pro-inflammatory cytokine protein expression in the jejunum and colon exhibited minimal change following fractionated radiotherapy. Conclusions: Pro-inflammatory cytokines play a key role in radiotherapy-induced gastrointestinal mucositis in the sub-acute onset setting. |
Keywords: | Intestinal Mucosa Animals Rats Inflammation RNA, Messenger Cytokines Immunohistochemistry Reverse Transcriptase Polymerase Chain Reaction Female Radiotherapy, Intensity-Modulated Mucositis |
Rights: | © 2010 Ong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1186/1748-717X-5-22 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1186/1748-717x-5-22 |
Appears in Collections: | Aurora harvest Medicine publications |
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hdl_59751.pdf | Published version | 766.55 kB | Adobe PDF | View/Open |
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