Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/60640
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Type: Journal article
Title: OAS1 polymorphisms are associated with susceptibility to West Nile encephalitis in horses
Author: Rios, J.
Fleming, J.
Bryant, U.
Carter, C.
Huber, J.
Long, M.
Spencer, T.
Adelson, D.
Citation: PLoS One, 2010; 5(5):1-9
Publisher: Public Library of Science
Issue Date: 2010
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Jonathan J. Rios, JoAnn G. W. Fleming, Uneeda K. Bryant, Craig N. Carter, John C. Huber, Jr., Maureen T. Long, Thomas E. Spencer and David L. Adelson
Abstract: West Nile virus, first identified within the United States in 1999, has since spread across the continental states and infected birds, humans and domestic animals, resulting in numerous deaths. Previous studies in mice identified the Oas1b gene, a member of the OAS/RNASEL innate immune system, as a determining factor for resistance to West Nile virus (WNV) infection. A recent case-control association study described mutations of human OAS1 associated with clinical susceptibility to WNV infection. Similar studies in horses, a particularly susceptible species, have been lacking, in part, because of the difficulty in collecting populations sufficiently homogenous in their infection and disease states. The equine OAS gene cluster most closely resembles the human cluster, with single copies of OAS1, OAS3 and OAS2 in the same orientation. With naturally occurring susceptible and resistant sub-populations to lethal West Nile encephalitis, we undertook a case-control association study to investigate whether, similar to humans (OAS1) and mice (Oas1b), equine OAS1 plays a role in resistance to severe WNV infection. We identified naturally occurring single nucleotide mutations in equine (Equus caballus) OAS1 and RNASEL genes and, using Fisher's Exact test, we provide evidence that mutations in equine OAS1 contribute to host susceptibility. Virtually all of the associated OAS1 polymorphisms were located within the interferon-inducible promoter, suggesting that differences in OAS1 gene expression may determine the host's ability to resist clinical manifestations associated with WNV infection.
Keywords: Fibroblasts; Animals; Horses; Humans; West Nile Fever; Horse Diseases; Genetic Predisposition to Disease; Luciferases; 2',5'-Oligoadenylate Synthetase; Interferons; Case-Control Studies; Haplotypes; Polymorphism, Single Nucleotide; Alleles; Promoter Regions, Genetic; Hep G2 Cells
Rights: Copyright: © 2010 Rios et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020097461
DOI: 10.1371/journal.pone.0010537
Appears in Collections:Molecular and Biomedical Science publications
Environment Institute publications

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