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|Title:||Endogenous glucagon-like peptide-1 slows gastric emptying in healthy subjects, attenuating postprandial glycemia|
|Citation:||Journal of Clinical Endocrinology and Metabolism, 2010; 95(1):215-221|
|Adam M. Deane, Nam Q. Nguyen, Julie E. Stevens, Robert J. L. Fraser, Richard H. Holloway, Laura K. Besanko, Carly Burgstad, Karen L. Jones, Marianne J. Chapman, Chris K. Rayner and Michael Horowitz|
|Abstract:||Introduction: The role of glucagon-like peptide-1 (GLP-1) in the regulation of gastric emptying is uncertain. The aim of this study was to determine the effects of endogenous GLP-1 on gastric emptying, glucose absorption, and glycemia in health. Methods: Ten healthy fasted subjects (eight males, two females; 48 ± 7 yr) received the specific GLP-1 antagonist, exendin(9-39) amide [ex(9-39)NH2] (300 pmol/kg · min iv), or placebo, between –30 and 180 min in a randomized, double-blind, crossover fashion. At 0 min, a mashed potato meal (~2600 kJ) containing 3 g 3-ortho-methyl-D-glucose (3-OMG) and labeled with 20 MBq 99mTechnetium-sulphur colloid was eaten. Gastric emptying, including the time taken for 50% of the meal to empty from the stomach (T50), blood glucose, plasma 3-OMG, and plasma insulin were measured. Results: Ex(9-39)NH2 accelerated gastric emptying [T50 ex(9-39)NH2, 68 ± 8 min, vs. placebo, 83 ± 7 min; P < 0.001] and increased the overall glycemic response to the meal [area under the curve (0-180 min) ex(9-39)NH2, 1540 ± 106 mmol/liter · min, vs. placebo, 1388 ± 90 mmol/liter · min; P < 0.02]. At 60 min, ex(9-39)NH2 increased the rise in glycemia [ex(9-39)NH2, 9.9 ± 0.5 mmol/liter, vs. placebo, 8.4 ± 0.5 mmol/liter; P < 0.01], plasma 3-OMG [ex(9-39)NH2, 0.25 ± 0.01 mmol/liter, vs. placebo, 0.21 ± 0.01 mmol/liter; P < 0.05], and plasma insulin [ex(9-39)NH2, 82 ± 13 mU/liter, vs. placebo, 59 ± 9 mU/liter; P < 0.05] concentrations. There was a close within-subject correlation between glycemia and gastric emptying [e.g. at 60 min, the increment in blood glucose and gastric emptying (T50); r = –0.89; P < 0.001]. Conclusion: GLP-1 plays a physiological role to slow gastric emptying in health, which impacts on glucose absorption and, hence, postprandial glycemia.|
|Keywords:||Humans; Hyperglycemia; Hormone Antagonists; Insulin; Blood Glucose; Peptide Fragments; Guanosine; Placebos; Cross-Over Studies; Double-Blind Method; Gastric Emptying; Adult; Middle Aged; Health; Female; Male; Glucagon-Like Peptide 1|
|Rights:||Copyright © 2010 by The Endocrine Society|
|Appears in Collections:||Anaesthesia and Intensive Care publications|
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