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|Title:||The density of human semen and the validation of weight as an indicator of volume: a multicentre study|
|Citation:||Reproductive Biology, 2010; 10(2):141-153|
|Publisher:||Towarzystwo Biologi Rozrodu|
|Phillip L. Matson, Kim Myssonski, Steven Yovich, Linda Morrison, Jacquelyn Irving, Hassan W. Bakos|
|Abstract:||A multi-centre study was undertaken to: a/ determine the density of human semen, and b/ assess the validity of measuring semen volume either volumetrically or gravimetrically. Semen samples from four clinical categories (azoospermia following vasectomy, azoospermia without vasectomy, oligozoospermia (<20×106/ml) and normozoospermia (>20×106/ml)) had similar densities (one-way ANOVA: F(3,180)=1.25, not significant), being close to 1.0 g/ml when taken to one decimal place. Measurement of semen volume was then made with either a graduated pipette or by weighing and assuming a density of 1 g/ml. A comparison of the two methods gave an excellent correlation, with a gradient of 1.0571 and a coefficient of determination (R2) of 0.98 (p<0.0001). However, it was noted that the aspiration of the ejaculate in to a graduated pipette underestimated the volume by approximately 0.2 ml, but in an inconsistent manner making the use of a set correction factor inappropriate. The estimation of volume to one decimal place by weighing the collection container before and after ejaculation, assuming a density of 1 g/ml, would seem to be a viable alternative although the density of a small number of samples may deviate from this assumption. Whilst the relatively small underestimation of volume with a pipette is unlikely to have clinical significance, the known reporting of inaccurate results by a laboratory is contrary to the philosophy and key principles of quality management.|
|Keywords:||semen analysis; ejaculate volume; semen density; semen weight; quality assurance|
|Rights:||Copyright © 2010 by the Society for Biology of Reproduction|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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