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https://hdl.handle.net/2440/62284
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dc.contributor.author | Dunning, K. | - |
dc.contributor.author | Cashman, K. | - |
dc.contributor.author | Russell, D. | - |
dc.contributor.author | Thompson, J. | - |
dc.contributor.author | Norman, R. | - |
dc.contributor.author | Robker, R. | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Biology of Reproduction, 2010; 83(6):909-918 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.issn | 1529-7268 | - |
dc.identifier.uri | http://hdl.handle.net/2440/62284 | - |
dc.description.abstract | Oocyte and embryo metabolism are closely linked with their subsequent developmental capacity. Lipids are a potent source of cellular energy, yet little is known about lipid metabolism during oocyte maturation and early embryo development. Generation of ATP from lipids occurs within mitochondria via beta-oxidation of fatty acids, with the rate-limiting step catalyzed by carnitine palmitoyl transferase I (CPT1B), a process also requiring carnitine. We sought to investigate the regulation and role of beta-oxidation during oocyte maturation and preimplantation development. Expression of Cpt1b mRNA, assessed by real-time RT-PCR in murine cumulus-oocyte complexes (COCs), increased following hormonal induction of oocyte maturation and ovulation in vivo with human chorionic gonadotropin (5 IU) and in embryos reaching the blastocyst stage. Beta-oxidation, measured by the production of 3H2O from [3H]palmitic acid, was significantly increased over that in immature COCs following induction of maturation in vitro with epidermal growth factor (3 ng/ml) and follicle-stimulating hormone (50 mIU/ml). The importance of lipid metabolism for oocyte developmental competence and early embryo development was demonstrated by assessing the rate of embryo development following inhibition or upregulation of beta-oxidation with etomoxir (an inhibitor of CPT1B) or l-carnitine, respectively. Inhibition of beta-oxidation during oocyte maturation or zygote cleavage impaired subsequent blastocyst development. In contrast, l-carnitine supplementation during oocyte maturation significantly increased beta-oxidation, improved developmental competence, and in the absence of a carbohydrate energy supply, significantly increased 2-cell cleavage. Thus, carnitine is an important cofactor for developing oocytes, and fatty acids are an important energy source for oocyte and embryo development. | - |
dc.description.statementofresponsibility | Kylie R. Dunning, Kara Cashman, Darryl L. Russell, Jeremy G. Thompson, Robert J. Norman, and Rebecca L. Robker | - |
dc.language.iso | en | - |
dc.publisher | Soc Study Reproduction | - |
dc.rights | © 2010 by the Society for the Study of Reproduction, Inc. | - |
dc.source.uri | http://dx.doi.org/10.1095/biolreprod.110.084145 | - |
dc.subject | beta-oxidation | - |
dc.subject | CPT1B | - |
dc.subject | embryo development | - |
dc.subject | fatty acid oxidation | - |
dc.subject | oocyte maturation | - |
dc.title | Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1095/biolreprod.110.084145 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Dunning, K. [0000-0002-0462-6479] | - |
dc.identifier.orcid | Russell, D. [0000-0002-4930-7658] | - |
dc.identifier.orcid | Thompson, J. [0000-0003-4941-7731] | - |
dc.identifier.orcid | Norman, R. [0000-0002-3118-3896] | - |
dc.identifier.orcid | Robker, R. [0000-0002-1538-4604] | - |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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