Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/62693
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Type: Journal article
Title: Microglial activation in the visual pathway in experimental glaucoma: Spatiotemporal characterization and correlation with axonal injury
Author: Ebneter, A.
Casson, R.
Wood, J.
Chidlow, G.
Citation: Investigative Ophthalmology & Visual Science, 2010; 51(12):6448-6460
Publisher: Assoc Research Vision Ophthalmology Inc
Issue Date: 2010
ISSN: 0146-0404
1552-5783
Statement of
Responsibility: 
Andreas Ebneter, Robert J. Casson, John P. M. Wood, and Glyn Chidlow
Abstract: Purpose. Glia are the main cellular CNS elements initiating defense mechanisms against destructive influences and promoting regenerative processes. The aim of the current work was to characterize the microglial response within the visual pathway in a rat model of experimental glaucoma and to correlate the microglial response with the severity of axonal degeneration. Methods. Experimental glaucoma was induced in each right eye of adult Sprague-Dawley rats by translimbal laser photocoagulation of the trabecular meshwork. Rats were subsequently killed at various times from 3 days to 6 weeks. Tissue sections were obtained from globes, optic nerves, chiasmata, and optic tracts for immunohistochemistry and toluidine blue staining. Results. This model of experimental glaucoma led to a marked activation of microglia in the retina, optic nerve, and tract. Indeed, microglial activity remained elevated, even after intraocular pressure returned to basal levels. It is postulated that this process accompanies ongoing axonal degeneration. The degree of activation in the optic nerve correlated with axonal damage. Activation was characterized by increased density and morphologic changes. Both major histocompatibility complex (MHC) class I and MHC class II surface proteins were persistently upregulated in optic nerves and localized to microglial cells; however, this did not correlate with any significant T-cell infiltration. Interestingly, MHC class II expression was not detected in the retina. Conclusions. The present data may have implications for the study of the pathology associated with the visual pathway in diseases such as glaucoma.
Keywords: Visual Pathways; Microglia; Axons; Optic Disk; Neutrophils; T-Lymphocytes; Animals; Rats; Rats, Sprague-Dawley; Optic Nerve Injuries; Glaucoma; Disease Models, Animal; Nerve Degeneration; Antigens, Surface; Fluorescent Antibody Technique, Indirect; Neutrophil Infiltration
Rights: Copyright © Association for Research in Vision and Ophthalmology
RMID: 0020101567
DOI: 10.1167/iovs.10-5284
Grant ID: http://purl.org/au-research/grants/nhmrc/508123
Appears in Collections:Opthalmology & Visual Sciences publications

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