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Type: Journal article
Title: Patients with chronic myeloid leukemia who maintain a complete molecular response after stopping imatinib treatment have evidence of persistent leukemia by DNA PCR
Author: Ross, D.
Branford, S.
Seymour, J.
Schwarer, A.
Arthur, C.
Bartley, P.
Slader, C.
Field, C.
Dang, P.
Filshie, R.
Mills, A.
Grigg, A.
Vaz de Melo, J.
Hughes, T.
Citation: Leukemia, 2010; 24(10):1719-1724
Publisher: Nature Publishing Group
Issue Date: 2010
ISSN: 0887-6924
Statement of
DM Ross, S Branford, JF Seymour, AP Schwarer, C Arthur, PA Bartley, C Slader, C Field, P Dang, RJ Filshie, AK Mills, AP Grigg, JV Melo and TP Hughes
Abstract: Around 40–50% of patients with chronic myeloid leukemia (CML) who achieve a stable complete molecular response (CMR; undetectable breakpoint cluster region-Abelson leukemia gene human homolog 1 (BCR–ABL1) mRNA) on imatinib can stop therapy and remain in CMR, at least for several years. This raises the possibility that imatinib therapy may not need to be continued indefinitely in some CML patients. Two possible explanations for this observation are (1) CML has been eradicated or (2) residual leukemic cells fail to proliferate despite the absence of ongoing kinase inhibition. We used a highly sensitive patient-specific nested quantitative PCR to look for evidence of genomic BCR–ABL1 DNA in patients who sustained CMR after stopping imatinib therapy. Seven of eight patients who sustained CMR off therapy had BCR–ABL1 DNA detected at least once after stopping imatinib, but none has relapsed (follow-up 12–41 months). BCR–ABL1 DNA levels increased in all of the 10 patients who lost CMR soon after imatinib cessation, whereas serial testing of patients in sustained CMR showed a stable level of BCR–ABL1 DNA. This more sensitive assay for BCR–ABL1 provides evidence that even patients who maintain a CMR after stopping imatinib may harbor residual leukemia. A search for intrinsic or extrinsic (for example, immunological) causes for this drug-free leukemic suppression is now indicated.
Keywords: chronic myeloid leukemia; minimal residual disease; PCR; imatinib mesylate
Rights: © 2010 Macmillan Publishers Limited. All rights reserved
RMID: 0020101178
DOI: 10.1038/leu.2010.185
Appears in Collections:Medicine publications

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