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Type: Journal article
Title: Diagnostic significance of Neospora caninum DNA detected by PCR in cattle serum
Author: McInnes, L.
Ryan, U.
O'Handley, R.
Sager, H.
Forshaw, D.
Palmer, D.
Citation: Veterinary Parasitology, 2006; 142(3-4):207-213
Publisher: Elsevier Science BV
Issue Date: 2006
ISSN: 0304-4017
Statement of
Linda M. McInnes, Una M. Ryan, Ryan O’Handley, Heinz Sager, David Forshaw and Dieter G. Palmer
Abstract: A nested PCR that successfully detected Neospora caninum DNA in serum of cattle was used for investigation of selected abortion cases and in a study of healthy pregnant cows at an abattoir. N. caninum DNA was not detected in serum from antibody positive dams that aborted due to N. caninum, but was present in serum of some antibody negative dams that aborted due to other causes. N. caninum DNA was also found in the serum of about half of the animals that aborted of undetermined cause, but was not detected in cow sera from two beef cattle herds in Western Australia with no recent history of abortion. In the abattoir study of 79 dams and their foetuses N. caninum DNA was found in serum of 3 dams and in material from 11 foetuses. The majority of the cows and all foetuses were antibody negative. Our findings suggest that there is no obvious relationship between the presence or absence of N. caninum DNA in serum and the presence of antibodies to N. caninum in dams, the presence of N. caninum DNA in foetuses or abortion due to N. caninum. This is the first report of the detection of N. caninum DNA in serum of cattle rather than the white blood cell fraction. It indicates the presence of free tachyzoites and/or parasite DNA in circulation. The results suggest that persistent infection in the absence of antibodies is a possible outcome of N. caninum infection. Infection of foetuses in the absence of antibodies supports the possibility of persistent infection due to immunotolerance to an early in utero infection. It is therefore important to test for N. caninum DNA as well as antibodies for the detection of exposed and/or infected animals. However, the presence or absence of N. caninum antibodies or DNA did not support nor exclude N. caninum as the cause of abortion. Additional criteria are required for a positive diagnosis of abortion caused by N. caninum.
Keywords: Neospora caninum; DNA; Antibodies; PCR; Cow; Foetus
Rights: Crown Copyright 2006 Published by Elsevier B.V. All rights reserved.
RMID: 0020108595
DOI: 10.1016/j.vetpar.2006.07.013
Appears in Collections:Animal and Veterinary Sciences publications

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