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Type: Journal article
Title: Prevalence and molecular characterization of Cryptosporidium spp. in dairy calves from 11 farms in Prince Edward Island, Canada
Author: Coklin, T.
Uehlinger, F.
Farber, J.
Barkema, H.
O'Handley, R.
Dixon, B.
Citation: Veterinary Parasitology, 2009; 160(3-4):323-326
Publisher: Elsevier Science BV
Issue Date: 2009
ISSN: 0304-4017
Statement of
Tatjana Coklin, Fabienne D. Uehlinger, Jeffrey M. Farber, Herman W. Barkema, Ryan M. O’Handley and Brent R. Dixon
Abstract: Cryptosporidium spp. are common intestinal protozoan parasites that infect a wide range of hosts, including humans and livestock, worldwide. The objective of this study was to determine the prevalence of Cryptosporidium spp. in dairy calves in Prince Edward Island, Canada, and the potential for transmission of this parasite between dairy calves and humans. Fecal samples were collected from 183 dairy calves from 11 farms in Prince Edward Island. The prevalence of Cryptosporidium spp. infections in these animals was determined by examining for the presence of oocysts in the fecal samples, using immunofluorescence microscopy. Molecular characterization was done using a nested-PCR protocol to amplify fragments of the Cryptosporidium heat-shock protein 70 gene, followed by DNA sequencing. Ten calves (6.2%), representing 4 out of 11 farms tested, were positive for Cryptosporidium spp. DNA sequence analysis on five PCR positive samples demonstrated that Cryptosporidium parvum was the only species present in the calves tested, suggesting that there is a potential risk of zoonotic transmission between dairy calves and humans in this region.
Keywords: Feces; Animals; Cattle; Humans; Cryptosporidium; Cryptosporidium parvum; Oocysts; Zoonoses; Cryptosporidiosis; Cattle Diseases; DNA, Protozoan; Prevalence; Risk Factors; Sequence Analysis, DNA; Phylogeny; Gene Amplification; Base Sequence; Molecular Sequence Data; Prince Edward Island; Female; HSP70 Heat-Shock Proteins
Rights: Crown Copyright © 2008 Published by Elsevier B.V. All rights reserved.
RMID: 0020108926
DOI: 10.1016/j.vetpar.2008.10.096
Appears in Collections:Animal and Veterinary Sciences publications

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