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https://hdl.handle.net/2440/64953
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Type: | Journal article |
Title: | Effects of Rho-kinase and Src protein tyrosine kinase inhibition on agonist-induced vasoconstriction of arteries and veins of the equine laminar dermis |
Author: | Robertson, T. Moore, J. Noschka, E. Lewis, T. Lewis, S. Peroni, J. |
Citation: | American Journal of Veterinary Research, 2007; 68(8):886-894 |
Publisher: | Amer Veterinary Medical Assoc |
Issue Date: | 2007 |
ISSN: | 0002-9645 1943-5681 |
Statement of Responsibility: | Tom P. Robertson, James N. Moore, Erik Noschka, Tristan H. Lewis, Stephen J. Lewis, John F. Peroni |
Abstract: | Objective — To determine the effects of inhibition of Rho-kinase or Src-family protein tyrosine kinases (srcPTK) on agonist-induced contractile responses in equine laminar arteries and veins. Sample Population — Laminar arteries and veins obtained from 13 adult mixed-breed horses. Procedures — Laminar vessels were mounted on myographs and exposed to phenylephrine (PE), 5-hydroxytryptamine (5-HT), prostaglandin F2α (PGF2α), and endothelin-1 (ET-1) with or without the Rho-kinase inhibitor Y-27632 (10μM), srcPTK inhibitor PP2 (10μM), or a negative control analogue for PP2 (PP3; 10μM). Results — Responses to PE were reduced by use of Y-27632 in laminar vessels (approx inhibition, 55%). However, Y-27632 reduced responses to 5-HT to a greater degree in veins than in arteries (approx inhibition of 55% and 35%, respectively). The Y-27632 also reduced responses of laminar veins to ET-1 by approximately 40% but had no effect on maximum responses of laminar arteries to ET-1, although a rightward shift in the concentration response curve was evident. Addition of PP2 reduced responses to PE, 5-HT, and PGF2α in laminar veins by approximately 40%, 60%, and 65%, respectively, compared with responses after the addition of PP3; PP2 had no effect on responses to ET-1. In laminar arteries, PP2 reduced 5-HT–induced contractions by approximately 50% but did not affect responses to PE or ET-1. Conclusions and Clinical Relevance — Results of the study were consistent with activation of Rho-kinase being important during agonist-induced constriction in laminar vessels, activation of srcPTK being an agonist-dependent event, and more prominent roles for Rhokinase and srcPTK in veins than in arteries. |
Keywords: | Foot Arteries Veins Animals Calcium Phenylephrine Amides Serotonin Pyrazoles Pyridines Pyrimidines src-Family Kinases Endothelin-1 Intracellular Signaling Peptides and Proteins Vasoconstrictor Agents Signal Transduction Vasoconstriction Dose-Response Relationship, Drug rho-Associated Kinases Protein Serine-Threonine Kinases |
Rights: | Copyright status unknown |
DOI: | 10.2460/ajvr.68.8.886 |
Published version: | http://dx.doi.org/10.2460/ajvr.68.8.886 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
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