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https://hdl.handle.net/2440/64995
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Type: | Journal article |
Title: | Effect of voltage-gated and capacitative calcium entry blockade on agonist-inducted construction of equine laminar blood vessels |
Author: | Peroni, J. Moore, J. Noschka, E. Lewis, T. Lewis, S. Robertson, T. |
Citation: | American Journal of Veterinary Research, 2007; 68(7):722-729 |
Publisher: | Amer Veterinary Medical Assoc |
Issue Date: | 2007 |
ISSN: | 0002-9645 1943-5681 |
Statement of Responsibility: | John F. Peroni, James N. Moore, Erik Noschka, Tristan H. Lewis, Stephen J. Lewis, Tom P. Robertson |
Abstract: | Objective—To characterize the relative contributions of voltage-gated and capacitative Ca²⁺ entry to agonist-induced contractions of equine laminar arteries and veins. Animals—16 adult mixed-breed horses. Procedures—Laminar arteries and veins were isolated and mounted on small vessel myographs for the measurement of isometric tension. Concentration-response curves were obtained for the vasoconstrictor agonists phenylephrine, 5-hydroxytryptamine (5-HT), prostaglandin F2α (PGF2α), and endothelin-1 (ET-1) either in the absence of extracellular Ca²⁺ or in the presence of the voltage-gated Ca²⁺ channel inhibitor diltiazem or the putative inhibitor of capacitative Ca2+ entry, trifluoromethylphenylimidazole. Results—In the absence of extracellular Ca2+, maximal responses of veins to 5-HT, phenylephrine, ET-1 and PGF2α were reduced by 80%, 50%, 50%, and 45%, respectively; responses of arteries to 5-HT, phenylephrine, and ET-1 were reduced by 95%, 90%, and 20%, respectively. Although diltiazem did not affect the maximal responses of veins to any agonist, responses of arteries to 5-HT, phenylephrine, and ET-1 were reduced by 40%, 50%, and 27%, respectively. Trifluoromethylphenylimidazole did not affect maximal responses of veins, but did reduce their contractile responses to low concentrations of ET-1 and PGF2α. Conclusions and Clinical Relevance—Results suggested that the contribution of extracellular Ca²⁺ to laminar vessel contractile responses differs between arteries and veins and also between contractile agonists, voltage-gated Ca²⁺ entry is more predominant in laminar arteries than in veins, and capacitative Ca²⁺ entry has a minor role in agonist-induced contractile responses of laminar veins. |
Keywords: | Muscle, Smooth, Vascular Hoof and Claw Animals Horses Calcium Phenylephrine Serotonin Imidazoles Diltiazem Dinoprost Endothelin-1 Adrenergic alpha-Agonists Calcium Channel Blockers Logistic Models Vasoconstriction In Vitro Techniques |
Rights: | Copyright status unknown |
DOI: | 10.2460/ajvr.68.7.722 |
Published version: | http://dx.doi.org/10.2460/ajvr.68.7.722 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 5 |
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