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https://hdl.handle.net/2440/66071
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Type: | Journal article |
Title: | Microheterogeneity and Coevolution: An Examination of rDNA sequence characteristics in Neoparamoeba pemaquidensis and its Prokinetoplastid Endosymbiont |
Author: | Caraguel, C. O'Kelly, C. Legendre, P. Frasca, S. Gast, R. Despres, B. Cawthorn, R. Greenwood, S. |
Citation: | The Journal of Eukaryotic Microbiology, 2007; 54(5):418-426 |
Publisher: | Soc Protozoologists |
Issue Date: | 2007 |
ISSN: | 1066-5234 1550-7408 |
Statement of Responsibility: | Charles G. B. Caraguel, Charles J. O’Kelly, Pierre Legendre, Salvatore Frasca Jr., Rebecca J. Gast, Béatrice M. Després, Richard J. Cawthorn, and Spencer J. Greenwood |
Abstract: | Neoparamoeba pemaquidensis, the etiological agent of amoebic gill disease, has shown surprising sequence variability among different copies of the 18S ribosomal RNA gene within an isolate. This intra-genomic microheterogeneity was confirmed and extended to an analysis of the internal transcribed spacer (ITS) region. High levels of intra-genomic nucleotide diversity (Pi=0.0201–0.0313) were found among sequenced ITS regions from individual host amoeba isolates. In contrast, the ITS region of its endosymbiont revealed significantly lower levels of intra-genomic nucleotide diversity (Pi=0.0028–0.0056) compared with the host N. pemaquidensis. Phylogenetic and ParaFit coevolution analyses involving N. pemaquidensis isolates and their respective endosymbionts confirmed a significant coevolutionary relationship between the two protists. The observation of non-shared microheterogeneity and coevolution emphasizes the complexity of the interactions between N. pemaquidensis and its obligate endosymbiont. |
Keywords: | Internal transcribed spacer intra-genomic variability parasome Perkinsiella amoebae-like organism 18S ribosomal RNA gene. |
Rights: | © 2007 The Author(s) |
DOI: | 10.1111/j.1550-7408.2007.00281.x |
Published version: | http://dx.doi.org/10.1111/j.1550-7408.2007.00281.x |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
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