Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/66205
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Type: Journal article
Title: Analysis of Biochemical Markers of Bone Metabolism in Asian Elephants (Elephas maximus)
Author: Kilgallon, C.
Flach, E.
Boardman, W.
Routh, A.
Strike, T.
Jackson, B.
Citation: Journal of Zoo and Wildlife Medicine, 2008; 39(4):527-536
Publisher: Amer Assoc Zoo Veterinarians
Issue Date: 2008
ISSN: 1042-7260
1937-2825
Statement of
Responsibility: 
Conor Kilgallon, Edmund Flach, Wayne Boardman, Andrew Routh, Taina Strike, and Brendan Jackson
Abstract: Two human enzyme immunoassays (EIA) and one radioimmunoassay (RIA) were validated and used to measure osteocalcin (OC), bone alkaline phosphatase (BAP), and the cross-linked telopeptide domain of type I collagen (ICTP), in serum from Asian elephants (Elephas maximus). Sera from four adult females sampled on 7 consecutive days were also analyzed to assess the existence and magnitude of intraindividual day-to-day variability of the serum concentration of these markers. Sample dilution curves were parallel with assay standard curves, which demonstrated that excellent cross reactivity existed between assay antibodies and elephants marker antigens. Statistically significant inverse correlations were found between age and concentrations of all three markers: BAP, r = −0.862 (P < 0.01); OC, r = −0.788 (P < 0.002); and ICTP, r = −0.848 (P < 0.01). Strong positive correlations were found between BAP and OC (r = 0.797, P < 0.01), OC and ICTP (r = 0.860, P < 0.01), and between BAP and ICTP (r = 0.958, P < 0.01). No statistically significant intraindividual variability was found over 7 days in the four adult females for any of the markers assessed (OC: P = 0.089; ICTP: P = 0.642; BAP: P = 0.146; n = 4 in each case). The overall coefficient of variability observed in this group of animals was 10.3%, 7.4%, and 5.5% for OC, BAP, and ICTP, respectively. These results suggest a potential role for biochemical markers of bone turnover in monitoring skeletal health and bone disease in Asian elephants.
Keywords: Bone markers; Asian elephants; osteodystrophy; immunoassay
Rights: Copyright 2008 by American Association of Zoo Veterinarians
RMID: 0020106235
DOI: 10.1638/2006-0024.1
Appears in Collections:Animal and Veterinary Sciences publications

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