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Type: Journal article
Title: Colonisation dynamics and virulence of two clonal groups of multidrug-resistant Escherichia coli isolated from dogs
Author: Sidjabat, H.
Chin, J.
Chapman, T.
Wu, K.
Ulett, G.
Ong, C.
Schembri, M.
Johnson, J.
Trott, D.
Citation: Microbes and Infection: a journal on infectious agents and host defenses, 2009; 11(1):100-107
Publisher: Editions Scientifiques Medicales Elsevier
Issue Date: 2009
ISSN: 1286-4579
Statement of
Hanna E. Sidjabat, James J.-C. Chin, Toni Chapman, Kent Wu, Glen C. Ulett, Cheryl Y. Ong, Mark A. Schembri, James R. Johnson, Darren J. Trott
Abstract: The study established the virulence potential of multidrug-resistant Escherichia coli (MDREC) isolates from nosocomial infections in hospitalised dogs. The isolates were resistant to fluoroquinolones, belonged to two distinct clonal groups (CG1 and CG2) and contained a plasmid-mediated AmpC (CMY-7) beta-lactamase. CG1 isolates (n=14) possessed two of 36 assayed extraintestinal virulence genes (iutA and traT) and belonged to phylogenetic group A, whereas CG2 isolates (n=19) contained four such genes (iutA, ibeA, fimH and kpsMT K5) and belonged to group D. In a mouse gastrointestinal tract colonisation model, colonisation by index CG1 strain C1 was transient, in contrast to the index CG2 strain C2b, which persisted up to 40days post-inoculation. In a mouse subcutaneous challenge model, both strains were less virulent than archetypal group B2 extraintestinal pathogenic E. coli (ExPEC) strain CFT073; strain C1 caused no systemic signs and strain C2b was lethal to only one of six mice. In a mouse urinary tract infection model, strain C2b colonised the mouse bladder over 2 logs higher compared to strain C1. Whilst both groups of canine MDREC appear less virulent than a reference human ExPEC strain, CG2 strains have greater capacity for colonisation and virulence.
Keywords: Virulence
Multidrug-resistant E. coli
Rights: Copyright © 2008 Published by Elsevier Masson SAS
DOI: 10.1016/j.micinf.2008.10.014
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Appears in Collections:Animal and Veterinary Sciences publications
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