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Type: Journal article
Title: Comparison of blood sirolimus, tacrolimus and everolimus concentrations measured by LC-MS/MS, HPLC-UV and immunoassay methods
Author: Sallustio, B.
Noll, B.
Morris, R.
Citation: Clinical Biochemistry, 2011; 44(2-3):231-236
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2011
ISSN: 0009-9120
Statement of
Benedetta C. Sallustio, Benjamin D. Noll, Raymond G. Morris
Abstract: An LC-MS/MS method was developed for simultaneous quantitation of tacrolimus, sirolimus and everolimus in whole blood, and compared to HPLC-UV and immunoassay methods.Blood (0.1mL) was analysed following solid-phase extraction and chromatographic resolution using a C18 column (45°C) and mobile phase of methanol/40mM ammonium acetate/glacial acetic acid (83/17/0.1) at 200μL/min, with positive electrospray ionisation and multiple reaction monitoring.Intra- and inter-day imprecision and inaccuracy were ≤12.2% over a 1.5-40μg/L calibration range. An external quality assurance programme confirmed acceptable inaccuracy and imprecision of the LC-MS/MS method, but highlighted problems with immunoassay quantitation, particularly for everolimus, showing a >30% bias in FPIA everolimus concentrations measured in pooled patient samples versus spiked drug-free whole blood.LC-MS/MS provides significant accuracy and precision advantages compared to HPLC and immunoassays. Discrepancies in everolimus concentrations measured by the Seradyn FPIA immunoassay require further investigation.
Keywords: LC-MS/MS; Immunoassays; Sirolimus; Tacrolimus; Everolimus
Rights: Crown copyright © 2010 Published by Elsevier Inc. All rights reserved.
RMID: 0020103215
DOI: 10.1016/j.clinbiochem.2010.10.005
Appears in Collections:Pharmacology publications

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