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https://hdl.handle.net/2440/66553
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Type: | Journal article |
Title: | Preclinical efficacy studies of Influenza A haemagglutinin precursor cleavage loop peptides as a potential vaccine |
Author: | Miller, D. Finnie, J. Bowden, T. Scholz, A. Oh, S. Kok, T. Burrell, C. Trinidad, L. Boyle, D. Li, P. |
Citation: | Journal of General Virology, 2011; 92(5):1152-1161 |
Publisher: | Soc General Microbiology |
Issue Date: | 2011 |
ISSN: | 0022-1317 1465-2099 |
Statement of Responsibility: | Darren S. Miller, John Finnie, Timothy R. Bowden, Anita C. Scholz, Sawyin Oh, Tuckweng Kok, Christopher J. Burrell, Lee Trinidad, David B. Boyle and Peng Li |
Abstract: | A universal influenza vaccine that does not require annual reformulation would have clear advantages over the currently approved seasonal vaccine. In this study, we combined the mucosal adjuvant alpha-galactosylceramide (αGalCer) and peptides designed across the highly conserved influenza precursor haemagglutinin (HA0) cleavage loop as a vaccine. Peptides designed across the HA0 of influenza A/H3N2 viruses, delivered to mice via the intranasal route with αGalCer as an adjuvant, provided 100 % protection following H3N2 virus challenge. Similarly, intranasal inoculation of peptides across the HA0 of influenza A/H5N1 with αGalCer completely protected mice against heterotypic challenge with H3N2 virus. Our data suggest that these peptide vaccines effectively inhibited subsequent influenza A/H3N2 virus replication. In contrast, only 20 % of mice vaccinated with αGalCer-adjuvanted peptides spanning the HA0 of H5N1 survived homologous viral challenge, possibly because the HA0 of this virus subtype is cleaved by intracellular furin-like enzymes. Results of these studies demonstrated that HA0 peptides adjuvanted with αGalCer have the potential to form the basis of a synthetic, intranasal influenza vaccine. |
Keywords: | Lung Animals Mice, Inbred BALB C Mice Orthomyxoviridae Infections Body Weight Galactosylceramides Protein Precursors Hemagglutinin Glycoproteins, Influenza Virus Vaccines, Subunit Influenza Vaccines Adjuvants, Immunologic Microscopy Histocytochemistry Viral Load Female Influenza A Virus, H3N2 Subtype Influenza A Virus, H5N1 Subtype Cross Protection |
Rights: | © 2011 SA Pathology |
DOI: | 10.1099/vir.0.028985-0 |
Published version: | http://dx.doi.org/10.1099/vir.0.028985-0 |
Appears in Collections: | Aurora harvest 5 Microbiology and Immunology publications |
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