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Type: Journal article
Title: Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries
Author: Do, T.
Stephens, C.
Townsend, K.
Wu, X.
Chapman, T.
Chin, J.
McCormick, B.
Bara, M.
Trott, D.
Citation: Australian Veterinary Journal, 2005; 83(5):293-299
Publisher: Australian Veterinary Assn
Issue Date: 2005
ISSN: 0005-0423
Statement of
T Do, C Stephens, K Townsend, X Wu, T Chapman, J Chin, B McCormick, M Bara and DJ Trott
Abstract: <h4>Objective</h4>To identify virulence genes in enterotoxigenic E coli (ETEC) isolates associated with diarrhoea in neonatal, 1 to 3 week-old and weaned pigs in southeast Queensland.<h4>Design</h4>Multiplex PCR and serotyping were applied to E coli isolates obtained over a 5-year period (1998-2002) from cases diagnosed at Toowoomba Veterinary Laboratory.<h4>Procedure</h4>A total of 126 isolates from 25 different Queensland piggeries were tested for haemolytic activity on 5% sheep blood agar and by multiplex PCR for the presence of five commonly recognised fimbrial (F4, F5, F6, F41 and F18) and three enterotoxin genes (STa, STb, LT). A subset of 62 representative isolates were serotyped by slide agglutination. For comparative purposes, multiplex PCR was also performed on the DNA of 31 ETEC isolates from 9 serotypes originating from piggeries in southern New South Wales.<h4>Results</h4>A total of 113 (89.7%) of the isolates from Queensland possessed ETEC virulence genes, including 14 of 15 isolates from neonatal pigs (93.3%), 18 of 23 isolates from 1 to 3 week old pigs (78.3%) and 81 of 88 isolates from weaned pigs (92.1%). F4:STa:STb:LT (serotype O149) was the most prevalent pathotype in neonatal and 1-3 week old pigs and F4:STa:STb:LT (serotype O149) and F18:STa:STb:LT (serotype O141) were most prevalent in weaned pigs. In comparison, isolates obtained from neonatal pigs from New South Wales belonged to a more diverse range of pathotypes and serotypes.<h4>Conclusion</h4>Multiplex PCR was a rapid and specific method for detecting the presence of ETEC virulence genes in porcine E coli isolates. For isolates obtained from cases of suspected colibacillosis in Queensland, growth of a heavy pure culture of haemolytic E coli was a sensitive prognostic indicator of the presence of ETEC virulence genes in the isolate. ETEC pathotypes and serotypes remained stable in Queensland piggeries over the five-year study period and appear to have changed little over the last three decades.
Keywords: Fimbriae, Bacterial
Animals, Newborn
Escherichia coli
Escherichia coli Infections
Swine Diseases
Fimbriae Proteins
Escherichia coli Proteins
Bacterial Toxins
Sensitivity and Specificity
Polymerase Chain Reaction
Genes, Bacterial
Time Factors
Rights: Copyright status unknown
DOI: 10.1111/j.1751-0813.2005.tb12745.x
Appears in Collections:Animal and Veterinary Sciences publications
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