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Type: Journal article
Title: Emergence and spread of two distinct clonal groups of multidrug-resistant Escherichia coli in a veterinary teaching hospital in Australia
Author: Sidjabat, H.
Townsend, K.
Lorentzen, M.
Gobius, K.
Fegan, N.
Chin, J.
Bettelheim, K.
Hanson, N.
Bensink, J.
Trott, D.
Citation: Journal of Medical Microbiology, 2006; 55(8):1125-1134
Publisher: Soc General Microbiology
Issue Date: 2006
ISSN: 0022-2615
Statement of
Hanna E. Sidjabat, Kirsty M. Townsend, Michael Lorentzen, Kari S. Gobius, Narelle Fegan, James J.-C. Chin, Karl A. Bettelheim, Nancy D. Hanson, John C. Bensink and Darren J. Trott
Abstract: Multidrug-resistant Escherichia coli (MDREC) expressing AmpC β-lactamases have emerged as a cause of opportunistic infections in dogs. Following a cluster of extraintestinal infections caused by two distinct clonal groups (CGs) of blaCMY-producing MDREC, a 12-month infection control study was undertaken at a veterinary teaching hospital in Brisbane, Australia. Swabs from the rectum of hospitalized dogs (n=780), hospital staff (n=16) and the hospital environment (n=220) were plated onto selective agar to obtain multidrug-resistant (MDR) coliforms. These were then tested by multiplex PCR for E. coli uspA, blaCMY and the class 1 integron-associated dfrA17-aadA5 gene cassette for rapid identification of MDREC CG 1 (positive for all three genes) and CG 2 (positive for uspA and blaCMY only). A total of 16.5 % of the dog rectal swabs and 4.1 % of the hospital environmental swabs yielded MDREC, and on the basis of multiplex PCR, PFGE and plasmid profiling, these were confirmed to belong to either CG 1 or CG 2. Both CG 1 and CG 2 isolates were obtained from clinical cases of extraintestinal infection and rectal swabs from hospitalized dogs over the same period of time, whereas only CG 1 isolates were obtained from the hospital environment. Both CGs were prevalent during the first 6 months, but only CG 2 was isolated during the second 6 months of the study. Two isolates obtained from rectal swabs of staff working in the hospital belonged to CG 2, with one of the isolates possessing the same REDP as nine isolates from dogs, including six isolates associated with cases of extraintestinal infection. CG 1 isolates belonged to E. coli serotypes O162 : H−, OR : H− or Ont : H−, whereas CG 2 isolates belonged to O153 : HR, OR : HR or OR : H34. These results confirm that in this particular outbreak, canine MDREC were highly clonal and CG 2 MDREC may colonize both humans and dogs.
Keywords: Rectum
Clone Cells
Escherichia coli
Escherichia coli Infections
Dog Diseases
Bacterial Proteins
Heat-Shock Proteins
Polymerase Chain Reaction
Hospitals, Animal
Environmental Monitoring
Drug Resistance, Multiple, Bacterial
Personnel, Hospital
Hospitals, Teaching
Molecular Epidemiology
Epidemiological Monitoring
Rights: © 2006 SGM
DOI: 10.1099/jmm.0.46598-0
Appears in Collections:Animal and Veterinary Sciences publications
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