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Type: Journal article
Title: Probiotic factors partially improve parameters of 5-fluorouracil-induced intestinal mucositis in rats
Author: Prisciandaro, L.
Geier, M.
Butler, R.
Cummins, A.
Howarth, G.
Citation: Cancer Biology & Therapy, 2011; 11(7):671-677
Publisher: Landes Bioscience
Issue Date: 2011
ISSN: 1538-4047
Statement of
Luca D. Prisciandaro, Mark S. Geier, Ross N. Butler, Adrian G. Cummins and Gordon S. Howarth
Abstract: Certain live bacteria have demonstrated preliminary indications of efficacy for the treatment of chemotherapy-induced intestinal mucositis. However, probiotic derived supernatants (SN) have yet to be investigated in the mucositis setting. We evaluated SN from Escherichia coli Nissle 1917 (EcN) and Lactobacillus fermentum BR11 (BR11) for their capacity to decrease 5-Fluorouracil (5-FU)-induced damage in vivo. Female Dark Agouti rats were gavaged with 1mL of either SN or vehicle daily (days 0-8) and intraperitoneally injected with 5-FU (150mg/kg) on day 5 to induce mucositis. On day 9, animals were culled and intestinal tissues collected. Significantly lower histological damage scores were apparent in the jejunum of 5-FU treated rats receiving SN compared to 5-FU controls. Myeloperoxidase levels in the jejunum of 5-FU treated rats were increased in vehicle and BR11 SN treatments compared to untreated controls, whereas no significant increase was observed after EcN SN treatment. 5-FU treatment significantly reduced villus height and crypt depth in the jejunum compared to normal controls; however no significant reduction in these parameters was observed in 5-FU treated rats receiving either SN. We conclude that bacterial SN, especially EcN, partially protect the intestine from 5-FU mucositis. Further studies are required to define specific mechanisms by which SN exert their beneficial effects.
Keywords: Intestinal Mucosa; Intestine, Small; Animals; Rats; Fluorouracil; Sucrase; Peroxidase; Mucins; Severity of Illness Index; Enzyme Activation; Probiotics; Female; Mucositis
Rights: Copyright status unknown
RMID: 0020105116
DOI: 10.4161/cbt.11.7.14896
Appears in Collections:Animal and Veterinary Sciences publications

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