Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Probiotic factors partially improve parameters of 5-fluorouracil-induced intestinal mucositis in rats|
|Citation:||Cancer Biology & Therapy, 2011; 11(7):671-677|
|Luca D. Prisciandaro, Mark S. Geier, Ross N. Butler, Adrian G. Cummins and Gordon S. Howarth|
|Abstract:||Certain live bacteria have demonstrated preliminary indications of efficacy for the treatment of chemotherapy-induced intestinal mucositis. However, probiotic derived supernatants (SN) have yet to be investigated in the mucositis setting. We evaluated SN from Escherichia coli Nissle 1917 (EcN) and Lactobacillus fermentum BR11 (BR11) for their capacity to decrease 5-Fluorouracil (5-FU)-induced damage in vivo. Female Dark Agouti rats were gavaged with 1mL of either SN or vehicle daily (days 0-8) and intraperitoneally injected with 5-FU (150mg/kg) on day 5 to induce mucositis. On day 9, animals were culled and intestinal tissues collected. Significantly lower histological damage scores were apparent in the jejunum of 5-FU treated rats receiving SN compared to 5-FU controls. Myeloperoxidase levels in the jejunum of 5-FU treated rats were increased in vehicle and BR11 SN treatments compared to untreated controls, whereas no significant increase was observed after EcN SN treatment. 5-FU treatment significantly reduced villus height and crypt depth in the jejunum compared to normal controls; however no significant reduction in these parameters was observed in 5-FU treated rats receiving either SN. We conclude that bacterial SN, especially EcN, partially protect the intestine from 5-FU mucositis. Further studies are required to define specific mechanisms by which SN exert their beneficial effects.|
|Keywords:||Intestinal Mucosa; Intestine, Small; Animals; Rats; Fluorouracil; Sucrase; Peroxidase; Mucins; Severity of Illness Index; Enzyme Activation; Probiotics; Female; Mucositis|
|Rights:||Copyright status unknown|
|Appears in Collections:||Animal and Veterinary Sciences publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.