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Type: Journal article
Title: Study of effector antigens of IBR virus on humoralimmunity
Author: Hemmatzadeh, F.
Keyvanfar, H.
Ahmadi, M.
Citation: Journal of Veterinary Research, 2007; 62(1):57-61
Publisher: University of Tehran
Issue Date: 2007
ISSN: 2008-2525
Statement of
Hemmatzadeh, F., Keyvanfar, H., Ahmadi, M.
Abstract: The objective of this study was to determine the protein pattern and antigenic structure of BHV-1. Ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by SN test. All samples were cultured on cell cultures and purified by ultracentrifugation. Purified isolates were electerophorsed using SDS-PAGE method and immunoblotted. Viral antigens were detected using anti BHV-1 bovine antiserum and molecular weight of the bands were determined. All samples showed identical band number and molecular weight except for one sample which revealed an additional 120-125 KDa band. Also immunoblotting data resulted in identified of twelve bands (150, 130, 115, 97, 77, 70, 55, 45, 40, 38, 32 and 25 KDa) suggesting involvement of these antigens in evoking humoral immune response in affected cattle.
Keywords: virus; IBR; SDS-PAGE; immunoblotting; antigen.
Rights: Copyright status unknown
RMID: 0020108292
Appears in Collections:Animal and Veterinary Sciences publications

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