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https://hdl.handle.net/2440/70362
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dc.contributor.author | Conn, S. | - |
dc.contributor.author | Gilliham, M. | - |
dc.contributor.author | Athman, A. | - |
dc.contributor.author | Schreiber, A. | - |
dc.contributor.author | Baumann, U. | - |
dc.contributor.author | Moller, I. | - |
dc.contributor.author | Cheng, N. | - |
dc.contributor.author | Stancombe, M. | - |
dc.contributor.author | Hirschi, K. | - |
dc.contributor.author | Webb, A. | - |
dc.contributor.author | Burton, R. | - |
dc.contributor.author | Kaiser, B. | - |
dc.contributor.author | Tyerman, S. | - |
dc.contributor.author | Leigh, R. | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | The Plant Cell, 2011; 23(1):240-257 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.issn | 1532-298X | - |
dc.identifier.uri | http://hdl.handle.net/2440/70362 | - |
dc.description.abstract | The physiological role and mechanism of nutrient storage within vacuoles of specific cell types is poorly understood. Transcript profiles from Arabidopsis thaliana leaf cells differing in calcium concentration ([Ca], epidermis <10 mM versus mesophyll >60 mM) were compared using a microarray screen and single-cell quantitative PCR. Three tonoplast-localized Ca2+ transporters, CAX1 (Ca2+/H+-antiporter), ACA4, and ACA11 (Ca2+-ATPases), were identified as preferentially expressed in Ca-rich mesophyll. Analysis of respective loss-of-function mutants demonstrated that only a mutant that lacked expression of both CAX1 and CAX3, a gene ectopically expressed in leaves upon knockout of CAX1, had reduced mesophyll [Ca]. Reduced capacity for mesophyll Ca accumulation resulted in reduced cell wall extensibility, stomatal aperture, transpiration, CO2 assimilation, and leaf growth rate; increased transcript abundance of other Ca2+ transporter genes; altered expression of cell wall–modifying proteins, including members of the pectinmethylesterase, expansin, cellulose synthase, and polygalacturonase families; and higher pectin concentrations and thicker cell walls. We demonstrate that these phenotypes result from altered apoplastic free [Ca2+], which is threefold greater in cax1/cax3 than in wild-type plants. We establish CAX1 as a key regulator of apoplastic [Ca2+] through compartmentation into mesophyll vacuoles, a mechanism essential for optimal plant function and productivity. | - |
dc.description.statementofresponsibility | Simon J. Conn, Matthew Gilliham, Asmini Athman, Andreas W. Schreiber, Ute Baumann, Isabel Moller, Ning-Hui Cheng, Matthew A. Stancombe, Kendal D. Hirschi, Alex A. R. Webb, Rachel Burton, Brent N. Kaiser, Stephen D. Tyerman, and Roger A. Leigh | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Plant Physiologists | - |
dc.rights | © 2011 American Society of Plant Biologists | - |
dc.source.uri | http://dx.doi.org/10.1105/tpc.109.072769 | - |
dc.subject | Cell Wall | - |
dc.subject | Vacuoles | - |
dc.subject | Arabidopsis | - |
dc.subject | Plant Leaves | - |
dc.subject | Calcium | - |
dc.subject | Antiporters | - |
dc.subject | Cation Transport Proteins | - |
dc.subject | Arabidopsis Proteins | - |
dc.subject | RNA, Plant | - |
dc.subject | Oligonucleotide Array Sequence Analysis | - |
dc.subject | Gene Expression Profiling | - |
dc.subject | Mutagenesis, Insertional | - |
dc.subject | Gene Expression Regulation, Plant | - |
dc.subject | Phenotype | - |
dc.subject | Mutation | - |
dc.subject | Plant Stomata | - |
dc.subject | Single-Cell Analysis | - |
dc.title | Cell-specific vacuolar calcium storage mediated by CAX1 regulates apoplastic calcium concentration, gas exchange, and plant productivity in arabidopsis | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1105/tpc.109.072769 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Gilliham, M. [0000-0003-0666-3078] | - |
dc.identifier.orcid | Schreiber, A. [0000-0002-9081-3405] | - |
dc.identifier.orcid | Baumann, U. [0000-0003-1281-598X] | - |
dc.identifier.orcid | Burton, R. [0000-0002-0638-4709] | - |
dc.identifier.orcid | Tyerman, S. [0000-0003-2455-1643] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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