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Type: Book chapter
Title: Chromosome Analysis in Invertebrates and Vertebrates
Author: Rowell, D.
Lim, S.
Grutzner, F.
Citation: Methods in Molecular Biology - Volume 772 - Molecular Methods for Evolutionary Genetics, 2011 / Orgogozo, V., Rockman, M. (ed./s), pp.13-36
Publisher: Springer
Publisher Place: United States
Issue Date: 2011
Series/Report no.: Methods in Molecular Biology ; 772
ISBN: 9781617792274
Statement of
David M. Rowell, Shu Ly Lim, and Frank Grutzner
Abstract: The revolution in molecular techniques over the last 30 years detracted from many traditional cytological techniques for examining basic biological problems. One of these casualties is the preparation of karyotypes and analysis of chromosomal structure, behaviour, and variation. Recent technology permitting the full sequencing of organisms has highlighted (but does not replace) the importance of understanding chromosomal constitution and karyotype structure, which underpin genome organisation. This chapter provides simple and straightforward protocols for the preparation of chromosome spreads from animals, and more advanced techniques for cell culture and chromosomal banding and hybridisation.
Keywords: Chromosomes; Invertebrate genetics; Cytological techniques; Karyology; Cell culture; Fluorescence in situ hybridisation
Rights: © Springer Science+Business Media, LLC 2011
RMID: 0020114812
DOI: 10.1007/978-1-61779-228-1_2
Appears in Collections:Genetics publications
Environment Institute Leaders publications

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