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|Title:||Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25|
|Citation:||Genomics, 1996; 37(2):229-233|
|Publisher:||ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS|
|Grossman, Alex ; Mittrücker, Hans-Willi ; Nicholl, Jillian ; Suzuki, Akira ; Chung, Stephen ; Antonio, Laarni ; Suggs, Sid ; Sutherland, Grant R. ; Siderovski, David P. ; Mak, Tak W.|
|Abstract:||The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of approximately 5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU-1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.|
|Keywords:||Hela Cells; Tumor Cells, Cultured; Chromosomes, Human, Pair 6; Animals; Humans; Mice; DNA-Binding Proteins; Transcription Factors; DNA, Complementary; In Situ Hybridization, Fluorescence; Chromosome Mapping; Cloning, Molecular; Binding Sites; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Interferon Regulatory Factors; Promoter Regions, Genetic|
|Appears in Collections:||Paediatrics publications|
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