Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/74922
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Gutnisky, C. | - |
dc.contributor.author | Morado, S. | - |
dc.contributor.author | Dalvit, G. | - |
dc.contributor.author | Thompson, J. | - |
dc.contributor.author | Cetica, P. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Reproduction Fertility and Development, 2013; 25(7):1026-1035 | - |
dc.identifier.issn | 1031-3613 | - |
dc.identifier.issn | 1448-5990 | - |
dc.identifier.uri | http://hdl.handle.net/2440/74922 | - |
dc.description.abstract | The aim of the present study was to determine the effect of altering glycolytic pathway activity during bovine IVM on the meiotic maturation rate, oxidative activity, mitochondrial activity and the mitochondrial distribution within oocytes. Glycolytic activity was manipulated using two inhibitors (ATP, NaF) and a stimulator (AMP) of key enzymes of the pathway. Inhibition of glucose uptake, lactate production and meiotic maturation rates was observed when media were supplemented with ATP or NaF. The addition of AMP to the maturation medium had no effect on glucose uptake, lactate production or meiotic maturation. In the absence of gonadotrophin supplementation, AMP stimulated both glucose uptake and lactate production. However, AMP also decreased cytoplasmic maturation, as determined by early cleavage. During IVM, oocyte oxidative and mitochondrial activity was observed to increase at 15 and 22 h maturation. Inhibiting glycolysis with ATP or NaF led to a reduced oxidative and mitochondrial pattern compared with the respective control groups. Stimulation of the pathway with AMP increased oxidative and mitochondrial activity. A progressive mitochondrial migration to the central area was observed during maturation; oocytes treated with ATP, NaF or AMP showed limited migration. The present study reveals the effects of altering glycolytic pathway activity in cumulus–oocyte complexes, revealing the link between glycolysis of the cumulus–oocyte complex and the oxidative and mitochondrial activity of the oocyte. | - |
dc.description.statementofresponsibility | Cynthia Gutnisky, Sergio Morado, Gabriel C. Dalvit, Jeremy G. Thompson and Pablo D. Cetica | - |
dc.language.iso | en | - |
dc.publisher | C S I R O Publishing | - |
dc.rights | Journal compilation copyright CSIRO 2012 | - |
dc.source.uri | http://dx.doi.org/10.1071/rd12193 | - |
dc.subject | cumulus–oocyte complex | - |
dc.subject | glycolysis | - |
dc.subject | maturation | - |
dc.subject | mitotracker green | - |
dc.subject | redox sensor red. | - |
dc.title | Glycolytic pathway activity: effect on IVM and oxidative metabolism of bovine oocytes | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1071/RD12193 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Thompson, J. [0000-0003-4941-7731] | - |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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File | Description | Size | Format | |
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hdl_74922.pdf | Accepted version | 528.75 kB | Adobe PDF | View/Open |
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