Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/75466
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Type: Journal article
Title: Effect of varying glucose and glucosamine concentration in vitro on mouse oocyte maturation and developmental competence
Author: Frank, L.
McDowall, M.
Russell, D.
Wang, X.
Inglis, D.
Gilchrist, R.
Thompson, J.
Citation: Reproduction Fertility and Development, 2013; 25(8):1095-1104
Publisher: C S I R O Publishing
Issue Date: 2013
ISSN: 1031-3613
1448-5990
Statement of
Responsibility: 
L. A. Frank, M. L. Sutton-McDowall, D. L. Russell, X. Wang, D. K. Feil, R. B. Gilchrist, and J. G. Thompson
Abstract: The effects of hyper- and hypo-glycaemic conditions during the in vitro maturation of mouse cumulus–oocyte complexes on developmental competence were examined, with an emphasis on the role of the hexosamine biosynthesis pathway. A low (1 mM) glucose concentration achieved optimal oocyte competence (3-fold higher blastocyst development rate compared with high (30 mM) glucose, P < 0.05). In addition, glucose supplementation during only the first hour after release from the follicle was necessary and sufficient to support oocyte maturation and embryo development to the blastocyst stage. Glucosamine (a known hyperglycaemic mimetic and specific activator of the hexosamine pathway) was able to substitute for glucose during this first hour, indicating that flux through the hexosamine pathway is essential for oocyte competence. In the absence of glucose throughout the maturation period, glucosamine was not able to increase developmental competence, and at higher concentrations (2.5 and 5 mM) had a detrimental effect on MII and blastocyst development rates, compared with controls (P < 0.05). These experiments underscore the importance of glucose metabolic pathways during in vitro maturation and support the concept that excess flux through the hexosamine pathway has detrimental consequences.
Keywords: culture medium; cumulus cells; embryo; hexosamine biosynthesis pathway.
Description: Published online: 7 November 2012
Rights: Journal compilation © CSIRO 2013
RMID: 0020123042
DOI: 10.1071/RD12275
Grant ID: http://purl.org/au-research/grants/nhmrc/453556
Appears in Collections:Obstetrics and Gynaecology publications

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