Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/75876
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Type: Journal article
Title: SAGA complex components and acetate repression in Aspergillus nidulans
Author: Georgakopoulos, P.
Lockington, R.
Kelly, J.
Citation: G3: Genes, Genomes, Genetics, 2012; 2(11):1357-1367
Publisher: Genetics Society of America
Issue Date: 2012
ISSN: 2160-1836
2160-1836
Statement of
Responsibility: 
Paraskevi Georgakopoulos, Robin A. Lockington, and Joan M. Kelly
Abstract: Alongside the well-established carbon catabolite repression by glucose and other sugars, acetate causes repression in Aspergillus nidulans. Mutations in creA, encoding the transcriptional repressor involved in glucose repression, also affect acetate repression, but mutations in creB or creC, encoding components of a deubiquitination system, do not. To understand the effects of acetate, we used a mutational screen that was similar to screens that uncovered mutations in creA, creB, and creC, except that glucose was replaced by acetate to identify mutations that were affected for repression by acetate but not by glucose. We uncovered mutations in acdX, homologous to the yeast SAGA component gene SPT8, which in growth tests showed derepression for acetate repression but not for glucose repression. We also made mutations in sptC, homologous to the yeast SAGA component gene SPT3, which showed a similar phenotype. We found that acetate repression is complex, and analysis of facA mutations (lacking acetyl CoA synthetase) indicates that acetate metabolism is required for repression of some systems (proline metabolism) but not for others (acetamide metabolism). Although plate tests indicated that acdX- and sptC-null mutations led to derepressed alcohol dehydrogenase activity, reverse-transcription quantitative real-time polymerase chain reaction showed no derepression of alcA or aldA but rather elevated induced levels. Our results indicate that acetate repression is due to repression via CreA together with metabolic changes rather than due to an independent regulatory control mechanism.
Keywords: Acetate repress
SAGA complex
carbon catabolite repression
crA
creB
creC
Rights: Copyright © 2012 Georgakopoulos et al.
DOI: 10.1534/g3.112.003913
Published version: http://dx.doi.org/10.1534/g3.112.003913
Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

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